Assembly of hepatitis delta virus particles

W. S. Ryu, M. Bayer, J. Taylor

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Hepatitis delta virus (HDV) is a subviral satellite of hepatitis B virus (HBV). Since the RNA genome of HDV can replicate in cultured cells in the absence of HBV, it has been suggested that the only helper function of HBV is to supply HBV coat proteins in the assembly process of HDV particles. To examine the factors involved in such virion assembly, we transiently cotransfected cells with various hepadnavirus constructs and cDNAs of HDV and analyzed the particles released into the medium. We report that the HDV genomic RNA and the delta antigen can be packaged by coat proteins of either HBV or the related hepadnavirus woodchuck hepatitis virus (WHV). Among the three co-carboxy-terminal coat proteins of WHV, the smallest form was sufficient to package the HDV genome; even in the absence of HDV RNA, the delta antigen could be packaged by this WHV coat protein. Also, of the two co-amino-terminal forms of the delta antigen, only the larger form was essential for packaging.

Original languageEnglish
Pages (from-to)2310-2315
Number of pages6
JournalJournal of Virology
Volume66
Issue number4
Publication statusPublished - 1992 Jan 1

Fingerprint

Hepatitis delta virus
Hepatitis Delta Virus
virion
Virion
Hepatitis B virus
Woodchuck hepatitis virus
Woodchuck Hepatitis B Virus
Hepatitis delta Antigens
Capsid Proteins
coat proteins
Hepadnaviridae
RNA
antigens
Genome
genome
Product Packaging
cultured cells
packaging
Cultured Cells
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Ryu, W. S., Bayer, M., & Taylor, J. (1992). Assembly of hepatitis delta virus particles. Journal of Virology, 66(4), 2310-2315.
Ryu, W. S. ; Bayer, M. ; Taylor, J. / Assembly of hepatitis delta virus particles. In: Journal of Virology. 1992 ; Vol. 66, No. 4. pp. 2310-2315.
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Ryu, WS, Bayer, M & Taylor, J 1992, 'Assembly of hepatitis delta virus particles', Journal of Virology, vol. 66, no. 4, pp. 2310-2315.

Assembly of hepatitis delta virus particles. / Ryu, W. S.; Bayer, M.; Taylor, J.

In: Journal of Virology, Vol. 66, No. 4, 01.01.1992, p. 2310-2315.

Research output: Contribution to journalArticle

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Ryu WS, Bayer M, Taylor J. Assembly of hepatitis delta virus particles. Journal of Virology. 1992 Jan 1;66(4):2310-2315.