Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress

Jin Ok Baek, Dashlkhumbe Byamba, Wen Hao Wu, Tae Gyun Kim, Mingeol Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Psoriasis is a chronic inflammatory skin disease that is thought to be related to oxidative stress. Much progress has been made in understanding the pathophysiology of psoriasis in relation to the immunologic and antioxidant systems. However, this progress has been hindered by the lack of an appropriate animal model for psoriasis. Recently, imiquimod (IQM)-induced psoriasislike cutaneous inflammation has been reported in mice and humans. We verified the usefulness of an IQM-induced mouse model in relation to the antioxidant system. BALB/C female mice at 8-10 weeks of age were treated with IQM cream in this study. We analyzed clinical and histopathological changes. Increased reactive oxygen species production was measured by glutathione assay. Levels of myeloperoxidase (MPO) and superoxide dismutase-1 (SOD1) were determined by western blotting and immunohistochemical analyses. The activity of SOD was measured by a SOD activity assay kit. Application of IQM-induced skin inflammation similar to psoriasis in clinical and histopathological aspects. Accumulation of immune cells was confirmed. Oxidative stress was increased, the antioxidant enzyme MPO levels were increased, and both SOD levels and activity were decreased. In conclusion, the IQM-induced mouse model showed an aberrant antioxidant system. Levels of MPO and oxidative stress were increased, and the level and activity of SOD were decreased. Since this model seemed to be an appropriate model for psoriasis, it can be used to further study the pathogenic role of redox imbalance in psoriasis.

Original languageEnglish
Pages (from-to)699-706
Number of pages8
JournalArchives of Dermatological Research
Volume304
Issue number9
DOIs
Publication statusPublished - 2012 Nov 1

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imiquimod
Psoriasis
Oxidative Stress
Antioxidants
Peroxidase
Inbred BALB C Mouse
Inflammation
Skin
Skin Diseases
Oxidation-Reduction
Glutathione
Reactive Oxygen Species
Animal Models
Western Blotting

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Baek, Jin Ok ; Byamba, Dashlkhumbe ; Wu, Wen Hao ; Kim, Tae Gyun ; Lee, Mingeol. / Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress. In: Archives of Dermatological Research. 2012 ; Vol. 304, No. 9. pp. 699-706.
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Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress. / Baek, Jin Ok; Byamba, Dashlkhumbe; Wu, Wen Hao; Kim, Tae Gyun; Lee, Mingeol.

In: Archives of Dermatological Research, Vol. 304, No. 9, 01.11.2012, p. 699-706.

Research output: Contribution to journalArticle

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