Assessment of Chlorella sp. as a potential feedstock for biological methane production

Jungsu Park, A. Naresh Kumar, Roent Dune A. Cayetano, Sang Hyoun Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Microalgal biomass sequestrates CO2 and is regarded as a promising renewable feedstock for anaerobic digestion because of its adequate carbohydrate content and lignin-free structure. This study optimizes the dilute-acid pretreatment of Chlorella sp. and subsequent biomethane production using response surface methodology and central composite design with temperature, pretreatment time and solid-to-liquid ratio as variables. A temperature of 64.1 °C, pretreatment time of 1.2 h, and a solid to liquid ratio of 0.29 were the optimal pretreatment conditions and resulted in a methane yield of 302.22 mL CH4/g COD and methane production rate of 110.04 mL CH4/g VSS-d. The severity factor of 1.5–1.6 was adequate to render the Chlorella sp. bioavailable for high methane recovery. The results obtained from the experiments conformed to those predicted by the model. This study effectively utilizes algal biomass for biomethane production and enables the possibility of scaled-up studies using a closed-loop approach.

Original languageEnglish
Article number123075
JournalBioresource technology
Publication statusPublished - 2020 Jun

Bibliographical note

Funding Information:
This work was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) funded by the Korean government ( MOTIE ) (grant number 20194110100010 ).

Publisher Copyright:
© 2020 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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