We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.
Bibliographical noteFunding Information:
The authors are grateful to Mr. J. Lim for the statistical analysis and Miss J.E. Kim for technical assistance. This work was presented in part at the annual meeting of the American Association for Cancer Research, San Francisco, April 1–4, 2000 and supported by a grant from 21C Frontier Functional Analysis of Human Genome Project (FG1-4-01), Ministry of Science and Technology, South Korea.
All Science Journal Classification (ASJC) codes
- Cancer Research