Association between the -159CT CD14 gene polymorphism and tuberculosis in a Korean population

Young Ae Kang, Hye Won Lee, Young Whan Kim, Sung Koo Han, Young Soo Shim, Jae Joon Yim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The aim of the present study was to confirm the association between the CD14 -159C/T polymorphism and tuberculosis in the Korean population and to elucidate the functional basis for this putative association. CD14 -159C/T genotypes were determined by PCR - restriction fragment length polymorphism analysis in 274 tuberculosis patients and 422 healthy controls. Recombinant CD14 promoter-luciferase reporter constructs, including the -159T or -159C allele, were transfected into K562 and BEAS-2B cells, and luciferase activities were measured and compared. Levels of serum sCD14 and interferon-γ secreted by peripheral blood mononuclear cells (PBMCs) were measured using enzyme-linked immunosorbent assay.The frequency of -159TT genotypes was higher in tuberculosis patients than in healthy controls. The promoter activity of the -159T allele was higher than that of the -159C allele. Serum sCD14 levels were higher among tuberculosis patients with -159TT genotypes than among those with -159CC genotypes and interferon-γ release by PBMCs was decreased in subjects with -159TT genotypes. In conclusion, the -159TT CD14 genotypes were associated with tuberculosis development in Koreans. This association might be a result of the higher promoter activity of the -159T allele, the higher level of sCD14, and the decreased interferon-γ secretion in subjects with -159TT genotypes.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume57
Issue number3
DOIs
Publication statusPublished - 2009 Dec 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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