Association of hepatitis C virus particles with immunoglobulin: A mechanism for persistent infection

S. H. Choo, H. S. So, J. M. Cho, Wang-Shick Ryu

Research output: Contribution to journalArticle

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Abstract

The physical properties of hepatitis C virus (HCV) particles were determined by ultracentrifugation on 20-60% isopycnic sucrose density gradients. We report that (i) two populations of HCV particles were found in the sera of patients with chronic HCV infection [at high density (1.186-1.213 g/ml) and at low density (1.099-1.127 g/ml)], (ii) virus particles with high density values were associated with immunoglobulin, and (iii) virus particles with low density values accumulated base changes within a hypervariable region (HVR) of the E2 envelope domain of the RNA genome. The results indicate that base changes within the HVR of E2 lead to the accumulation of immunoglobulin-free virus particles. Therefore, these findings imply that persistent HCV infection is established as a consequence of sequence variation in the E2 envelope domain.

Original languageEnglish
Pages (from-to)2337-2341
Number of pages5
JournalJournal of General Virology
Volume76
Issue number9
DOIs
Publication statusPublished - 1995 Jan 1

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Hepacivirus
Virion
Immunoglobulin A
Infection
Virus Diseases
Immunoglobulins
Ultracentrifugation
Chronic Hepatitis C
Sucrose
Genome
RNA
Serum
Population

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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abstract = "The physical properties of hepatitis C virus (HCV) particles were determined by ultracentrifugation on 20-60{\%} isopycnic sucrose density gradients. We report that (i) two populations of HCV particles were found in the sera of patients with chronic HCV infection [at high density (1.186-1.213 g/ml) and at low density (1.099-1.127 g/ml)], (ii) virus particles with high density values were associated with immunoglobulin, and (iii) virus particles with low density values accumulated base changes within a hypervariable region (HVR) of the E2 envelope domain of the RNA genome. The results indicate that base changes within the HVR of E2 lead to the accumulation of immunoglobulin-free virus particles. Therefore, these findings imply that persistent HCV infection is established as a consequence of sequence variation in the E2 envelope domain.",
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Association of hepatitis C virus particles with immunoglobulin : A mechanism for persistent infection. / Choo, S. H.; So, H. S.; Cho, J. M.; Ryu, Wang-Shick.

In: Journal of General Virology, Vol. 76, No. 9, 01.01.1995, p. 2337-2341.

Research output: Contribution to journalArticle

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