Asymmetrical barcode adapterassisted recovery of duplicate reads and error correction strategy to detect rare mutations in circulating tumor DNA

Jinwoo Ahn, Hwang Byungjin, Ha Young Kim, Jang Hoon, Hwang Phill Kim, Sae Won Han, Tae You Kim, Ji Hyun Lee, Duhee Bang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Deep sequencing is required for the highly sensitive detection of rare variants in circulating tumor DNA (ctDNA). However, there remains a challenge for improved sensitivity and specificity. Maximum-depth sequencing is crucial to detect minority mutations that contribute to cancer progression. The associated costs become prohibitive as the numbers of targets and samples increase. We describe the targeted sequencing of KRAS in plasma samples using an efficient barcoding approach to recover discarded reads marked as duplicates. Combined with an error-removal strategy, we anticipate that our method could improve the accuracy of genotype calling, especially to detect rare mutations in the monitoring of ctDNA.

Original languageEnglish
Article number46678
JournalScientific reports
Volume7
DOIs
Publication statusPublished - 2017 May 2

All Science Journal Classification (ASJC) codes

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