AtKPNB1, an Arabidopsis importin-β protein, is downstream of the RING E3 ubiquitin ligase AtAIRP1 in the ABA-mediated drought stress response

Tae Rin Oh, Seong Gwan Yu, Hee Woong Yang, Jong Hum Kim, Woo Taek Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Main conclusion: AtKPNB1, an Arabidopsis importin-β protein, was regulated by AtAIRP1 E3 ubiquitin ligase, which intensified the ABA-mediated drought stress response. Abstract: As an early step in the abscisic acid (ABA)-mediated drought response, the ABA signal is transduced into the nucleus, and thus the nuclear transport system is crucially involved in the drought stress response. AtKPNB1, an importin-β protein, which is a core component of nuclear transport, was previously reported to be a negative factor in the ABA-mediated drought stress response (Luo et al. Luo et al., Plant J 75:377–389, 2013). Here, we report that AtAIPR1, an Arabidopsis RING-type E3 ubiquitin (Ub) ligase, interacted with and ubiquitinated AtKPNB1. A null mutation of AtKPNB1 suppressed the ABA-insensitive germination phenotype of atairp1 mutant seedlings as compared to that of the wild-type plants. Furthermore, the ABA-insensitive stomatal closure and drought-susceptible phenotypes of atairp1 were rescued in atairp1atkpnb1 double mutant progeny, indicating that AtKPNB1 functions downstream of AtAIRP1. These data suggest that AtAIRP1 regulates the ABA-mediated drought response in Arabidopsis via ubiquitination of AtKPNB1.

Original languageEnglish
Article number93
JournalPlanta
Volume252
Issue number5
DOIs
Publication statusPublished - 2020 Nov 1

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation (Mid-Career Researcher Program, Project No. 2017R1A2B2006750/2020R1A2B5B02001590 and Basic Science Research Program, Project Number 2018R1A6A1A03025607, Republic of Korea) to W.T.K. S.G.Y was the recipient of a Hyundai Motor Chung Mong-Koo Foundation Scholarship.

Funding Information:
This work was supported by grants from the National Research Foundation (Mid-Career Researcher Program, Project No. 2017R1A2B2006750/2020R1A2B5B02001590 and Basic Science Research Program, Project Number 2018R1A6A1A03025607, Republic of Korea) to W.T.K. S.G.Y was the recipient of a Hyundai Motor Chung Mong-Koo Foundation Scholarship.

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'AtKPNB1, an Arabidopsis importin-β protein, is downstream of the RING E3 ubiquitin ligase AtAIRP1 in the ABA-mediated drought stress response'. Together they form a unique fingerprint.

Cite this