ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors

Jae K. Ryu, Jean Kim, Sang H. Choi, Young J. Oh, Yong B. Lee, Seung U. Kim, Byung K. Jin

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Abstract

The present study examined the in vivo effects of ATP on the striatum of Sprague-Dawley rats. Intrastriatal administration of ATP produced dose-dependent striatal lesions as confirmed by cresyl violet staining. Additional immunostaining using neuronal nuclear protein (NeuN), OX-42 and GFAP antibodies revealed that ATP caused death of both neurons and glial cells. The nonmetabolizable ATP analogue ATPγS and P2X receptor agonist α,β-methylene ATP (α,β-MeATP) mimicked ATP effects, whereas either P2Y receptor agonist ADP or PI receptor agonist adenosine did not. The P2 receptor antagonist reactive blue 2, but not pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid (PPADS) attenuated ATP-induced striatal injury. These results suggest that intrastriatal administration of ATP causes P2X receptor-mediated cell death in the striatum and support the hypothesis that extracellular ATP can be an important mediator of neuropathological events of brain injuries.

Original languageEnglish
Pages (from-to)1611-1615
Number of pages5
JournalNeuroReport
Volume13
Issue number13
DOIs
Publication statusPublished - 2002 Sep 16

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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    Ryu, J. K., Kim, J., Choi, S. H., Oh, Y. J., Lee, Y. B., Kim, S. U., & Jin, B. K. (2002). ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors. NeuroReport, 13(13), 1611-1615. https://doi.org/10.1097/00001756-200209160-00008