ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors

Jae K. Ryu, Jean Kim, Sang H. Choi, Young Jun Oh, Yong B. Lee, Seung U. Kim, Byung K. Jin

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The present study examined the in vivo effects of ATP on the striatum of Sprague-Dawley rats. Intrastriatal administration of ATP produced dose-dependent striatal lesions as confirmed by cresyl violet staining. Additional immunostaining using neuronal nuclear protein (NeuN), OX-42 and GFAP antibodies revealed that ATP caused death of both neurons and glial cells. The nonmetabolizable ATP analogue ATPγS and P2X receptor agonist α,β-methylene ATP (α,β-MeATP) mimicked ATP effects, whereas either P2Y receptor agonist ADP or PI receptor agonist adenosine did not. The P2 receptor antagonist reactive blue 2, but not pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid (PPADS) attenuated ATP-induced striatal injury. These results suggest that intrastriatal administration of ATP causes P2X receptor-mediated cell death in the striatum and support the hypothesis that extracellular ATP can be an important mediator of neuropathological events of brain injuries.

Original languageEnglish
Pages (from-to)1611-1615
Number of pages5
JournalNeuroReport
Volume13
Issue number13
DOIs
Publication statusPublished - 2002 Sep 16

Fingerprint

Adenosine Triphosphate
Corpus Striatum
Cibacron Blue F 3GA
Purinergic P2Y12 Receptors
Purinergic P1 Receptor Agonists
Pyridoxal Phosphate
Nuclear Proteins
Neuroglia
Brain Injuries
Sprague Dawley Rats
Cell Death
Staining and Labeling
Neurons
Acids
Antibodies
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Ryu, J. K., Kim, J., Choi, S. H., Oh, Y. J., Lee, Y. B., Kim, S. U., & Jin, B. K. (2002). ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors. NeuroReport, 13(13), 1611-1615. https://doi.org/10.1097/00001756-200209160-00008
Ryu, Jae K. ; Kim, Jean ; Choi, Sang H. ; Oh, Young Jun ; Lee, Yong B. ; Kim, Seung U. ; Jin, Byung K. / ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors. In: NeuroReport. 2002 ; Vol. 13, No. 13. pp. 1611-1615.
@article{9b37a33dc3bc46f7822165fb42765cce,
title = "ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors",
abstract = "The present study examined the in vivo effects of ATP on the striatum of Sprague-Dawley rats. Intrastriatal administration of ATP produced dose-dependent striatal lesions as confirmed by cresyl violet staining. Additional immunostaining using neuronal nuclear protein (NeuN), OX-42 and GFAP antibodies revealed that ATP caused death of both neurons and glial cells. The nonmetabolizable ATP analogue ATPγS and P2X receptor agonist α,β-methylene ATP (α,β-MeATP) mimicked ATP effects, whereas either P2Y receptor agonist ADP or PI receptor agonist adenosine did not. The P2 receptor antagonist reactive blue 2, but not pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid (PPADS) attenuated ATP-induced striatal injury. These results suggest that intrastriatal administration of ATP causes P2X receptor-mediated cell death in the striatum and support the hypothesis that extracellular ATP can be an important mediator of neuropathological events of brain injuries.",
author = "Ryu, {Jae K.} and Jean Kim and Choi, {Sang H.} and Oh, {Young Jun} and Lee, {Yong B.} and Kim, {Seung U.} and Jin, {Byung K.}",
year = "2002",
month = "9",
day = "16",
doi = "10.1097/00001756-200209160-00008",
language = "English",
volume = "13",
pages = "1611--1615",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "13",

}

Ryu, JK, Kim, J, Choi, SH, Oh, YJ, Lee, YB, Kim, SU & Jin, BK 2002, 'ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors', NeuroReport, vol. 13, no. 13, pp. 1611-1615. https://doi.org/10.1097/00001756-200209160-00008

ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors. / Ryu, Jae K.; Kim, Jean; Choi, Sang H.; Oh, Young Jun; Lee, Yong B.; Kim, Seung U.; Jin, Byung K.

In: NeuroReport, Vol. 13, No. 13, 16.09.2002, p. 1611-1615.

Research output: Contribution to journalArticle

TY - JOUR

T1 - ATP-induced in vivo neurotoxicity in the rat striatum via P2 receptors

AU - Ryu, Jae K.

AU - Kim, Jean

AU - Choi, Sang H.

AU - Oh, Young Jun

AU - Lee, Yong B.

AU - Kim, Seung U.

AU - Jin, Byung K.

PY - 2002/9/16

Y1 - 2002/9/16

N2 - The present study examined the in vivo effects of ATP on the striatum of Sprague-Dawley rats. Intrastriatal administration of ATP produced dose-dependent striatal lesions as confirmed by cresyl violet staining. Additional immunostaining using neuronal nuclear protein (NeuN), OX-42 and GFAP antibodies revealed that ATP caused death of both neurons and glial cells. The nonmetabolizable ATP analogue ATPγS and P2X receptor agonist α,β-methylene ATP (α,β-MeATP) mimicked ATP effects, whereas either P2Y receptor agonist ADP or PI receptor agonist adenosine did not. The P2 receptor antagonist reactive blue 2, but not pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid (PPADS) attenuated ATP-induced striatal injury. These results suggest that intrastriatal administration of ATP causes P2X receptor-mediated cell death in the striatum and support the hypothesis that extracellular ATP can be an important mediator of neuropathological events of brain injuries.

AB - The present study examined the in vivo effects of ATP on the striatum of Sprague-Dawley rats. Intrastriatal administration of ATP produced dose-dependent striatal lesions as confirmed by cresyl violet staining. Additional immunostaining using neuronal nuclear protein (NeuN), OX-42 and GFAP antibodies revealed that ATP caused death of both neurons and glial cells. The nonmetabolizable ATP analogue ATPγS and P2X receptor agonist α,β-methylene ATP (α,β-MeATP) mimicked ATP effects, whereas either P2Y receptor agonist ADP or PI receptor agonist adenosine did not. The P2 receptor antagonist reactive blue 2, but not pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid (PPADS) attenuated ATP-induced striatal injury. These results suggest that intrastriatal administration of ATP causes P2X receptor-mediated cell death in the striatum and support the hypothesis that extracellular ATP can be an important mediator of neuropathological events of brain injuries.

UR - http://www.scopus.com/inward/record.url?scp=0037120277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037120277&partnerID=8YFLogxK

U2 - 10.1097/00001756-200209160-00008

DO - 10.1097/00001756-200209160-00008

M3 - Article

C2 - 12352612

AN - SCOPUS:0037120277

VL - 13

SP - 1611

EP - 1615

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 13

ER -