Atrophy of brown adipocytes in the adult mouse causes transformation into white adipocyte-like cells

Dae Whan Kim, Beom Sue Kim, Hee Seok Kwon, Chan Gil Kim, Han Woong Lee, Woong Hwan Choi, Chul Geun Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Adipose tissue is an important endocrine regulator of glucose metabolism and energy homeostasis. Researches have focused on this tissue not only as a target for pharmacotherapy of obesity and insulin resistance but also as an endocrine tissue with leptin secretion and high insulin sensitivity. Brown adipose tissue (BAT) additionally plays a unique role in thermoregulation through the mitochondrial uncoupling protein 1 (UCP1), which uncouples oxidative phosphorylation. As a genetic tissue ablation model of BAT, we made transgenic mice expressing herpes simplex virus thymidine kinase (HSV-TK) driven by the brown adipocyte-specific UCP1 minimal regulatory element. The HSV-TK transgene was expressed specifically in BAT and more than 35% increase of apoptosis was induced by ganciclovir (GCV) treatment. Nevertheless, the expression level was not high enough to induce BAT ablation in GCV-treated adult mice. Importantly, however, we found that brown adipocytes in the periphery of interscapular BAT were transformed into white adipocyte-like unilocular cells. These cells express white adipocyte-specific leptin protein but are different in the ultrastructure of mitochondria from classical white adipocytes. Our data indicates that atrophy of BAT causes transformation into white adipocyte-like cells in the adult mouse and also suggests that further molecular understanding of adipocyte plasticity using our transgenic mouse model might be beneficial for the development of anti-obesity/antidiabetic therapies.

Original languageEnglish
Pages (from-to)518-526
Number of pages9
JournalExperimental and Molecular Medicine
Volume35
Issue number6
DOIs
Publication statusPublished - 2003 Dec 31

Fingerprint

White Adipocytes
Brown Adipocytes
Brown Adipose Tissue
Atrophy
Tissue
Ganciclovir
Thymidine Kinase
Simplexvirus
Leptin
Transgenic Mice
Insulin Resistance
Obesity
Ablation
Viruses
Body Temperature Regulation
Oxidative Phosphorylation
Transgenes
Hypoglycemic Agents
Adipocytes
Energy Metabolism

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Kim, Dae Whan ; Kim, Beom Sue ; Kwon, Hee Seok ; Kim, Chan Gil ; Lee, Han Woong ; Choi, Woong Hwan ; Kim, Chul Geun. / Atrophy of brown adipocytes in the adult mouse causes transformation into white adipocyte-like cells. In: Experimental and Molecular Medicine. 2003 ; Vol. 35, No. 6. pp. 518-526.
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Atrophy of brown adipocytes in the adult mouse causes transformation into white adipocyte-like cells. / Kim, Dae Whan; Kim, Beom Sue; Kwon, Hee Seok; Kim, Chan Gil; Lee, Han Woong; Choi, Woong Hwan; Kim, Chul Geun.

In: Experimental and Molecular Medicine, Vol. 35, No. 6, 31.12.2003, p. 518-526.

Research output: Contribution to journalArticle

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