Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films

H. J. Son, D. W. Han, H. H. Kim, H. J. Kim, I. S. Lee, J. K. Kim, J. C. Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 μg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 μg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 μg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.

Original languageEnglish
Pages (from-to)291-294
Number of pages4
JournalKey Engineering Materials
Volume288-289
Publication statusPublished - 2005 Dec 1

Fingerprint

Military electronic countermeasures
Fibroblasts
Fibronectins
Collagen
Tetrazolium Salts
Bioelectric potentials
Cell proliferation
Cell growth
Scaffolds (biology)
Tissue engineering
polylactic acid-polyglycolic acid copolymer
Salts
Water

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Son, H. J., Han, D. W., Kim, H. H., Kim, H. J., Lee, I. S., Kim, J. K., & Park, J. C. (2005). Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films. Key Engineering Materials, 288-289, 291-294.
Son, H. J. ; Han, D. W. ; Kim, H. H. ; Kim, H. J. ; Lee, I. S. ; Kim, J. K. ; Park, J. C. / Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films. In: Key Engineering Materials. 2005 ; Vol. 288-289. pp. 291-294.
@article{455355b84c924ca6bafbc67e2b802cfd,
title = "Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films",
abstract = "In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 μg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 μg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 μg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.",
author = "Son, {H. J.} and Han, {D. W.} and Kim, {H. H.} and Kim, {H. J.} and Lee, {I. S.} and Kim, {J. K.} and Park, {J. C.}",
year = "2005",
month = "12",
day = "1",
language = "English",
volume = "288-289",
pages = "291--294",
journal = "Key Engineering Materials",
issn = "1013-9826",
publisher = "Trans Tech Publications",

}

Son, HJ, Han, DW, Kim, HH, Kim, HJ, Lee, IS, Kim, JK & Park, JC 2005, 'Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films', Key Engineering Materials, vol. 288-289, pp. 291-294.

Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films. / Son, H. J.; Han, D. W.; Kim, H. H.; Kim, H. J.; Lee, I. S.; Kim, J. K.; Park, J. C.

In: Key Engineering Materials, Vol. 288-289, 01.12.2005, p. 291-294.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films

AU - Son, H. J.

AU - Han, D. W.

AU - Kim, H. H.

AU - Kim, H. J.

AU - Lee, I. S.

AU - Kim, J. K.

AU - Park, J. C.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 μg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 μg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 μg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.

AB - In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 μg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 μg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 μg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.

UR - http://www.scopus.com/inward/record.url?scp=34249746927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249746927&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:34249746927

VL - 288-289

SP - 291

EP - 294

JO - Key Engineering Materials

JF - Key Engineering Materials

SN - 1013-9826

ER -

Son HJ, Han DW, Kim HH, Kim HJ, Lee IS, Kim JK et al. Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films. Key Engineering Materials. 2005 Dec 1;288-289:291-294.