Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films

H. J. Son, D. W. Han, H. H. Kim, H. J. Kim, I. S. Lee, J. K. Kim, J. C. Park

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Abstract

In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 μg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 μg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 μg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.

Original languageEnglish
Pages (from-to)291-294
Number of pages4
JournalKey Engineering Materials
Volume288-289
Publication statusPublished - 2005 Dec 1

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All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Son, H. J., Han, D. W., Kim, H. H., Kim, H. J., Lee, I. S., Kim, J. K., & Park, J. C. (2005). Attachment and proliferation of human dermal fibroblasts onto ECM-lmmobilized PLGA films. Key Engineering Materials, 288-289, 291-294.