Autodisplay of streptavidin

Min Park, Joachim Jose, Sarah Thömmes, Jo Il Kim, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on a single E. coli cell was evaluated with densitometric analysis. The biotin-binding activity of the autodisplayed streptavidin was estimated after treatment with fluorescently labeled biotin by fluorescence microscopy and flow cytometry. The biotin-binding activity of the E. coli with autodisplayed streptavidin was compared with the activity of streptavidin immobilized on magnetic beads. Finally, the outer membrane presenting autodisplayed streptavidin was isolated and layered on a 96-well microplate for an immunoassay.

Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalEnzyme and Microbial Technology
Volume48
Issue number4-5
DOIs
Publication statusPublished - 2011 Apr 7

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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    Park, M., Jose, J., Thömmes, S., Kim, J. I., Kang, M. J., & Pyun, J. C. (2011). Autodisplay of streptavidin. Enzyme and Microbial Technology, 48(4-5), 307-311. https://doi.org/10.1016/j.enzmictec.2010.12.006