Autofocusing and edge detection schemes in cell volume measurements with quantitative phase microscopy

Seungrag Lee, Ji Yong Lee, Wenzhong Yang, Dug Young Kim

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We have proposed and demonstrated a very sensitive volume measurement scheme for a live cell with a quantitative phase microscopy (QPM) utilizing auto-focusing and numerical edge detection schemes. An auto-focusing technique with two different focus measures is applied to find the focus dependent errors in our live cell volume measurement system. The volume of a polystyrene bead sample with 3m diameter has been measured for the validity test of our proposed method. We have shown that a small displacement of an object from its focusing position can cause a large volume error. A numerical edge detection technique is also used to accurately resolve the boundary between a cell and its suspension medium. We have applied this method to effectively suppress errors by the surrounding medium of a single red blood cell (RBC).

Original languageEnglish
Pages (from-to)6476-6486
Number of pages11
JournalOptics Express
Volume17
Issue number8
DOIs
Publication statusPublished - 2009 Apr 13

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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