Automated laser guidance of neuronal growth cones using a spatial light modulator

David J. Carnegie, Tomás Čižmár, Jörg Baumgartl, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.

Original languageEnglish
Pages (from-to)682-692
Number of pages11
JournalJournal of Biophotonics
Volume2
Issue number11
DOIs
Publication statusPublished - 2009 Nov 1

Fingerprint

laser guidance
Growth Cones
Electronic guidance systems
light modulators
Cones
cones
Lasers
Laser beams
Light
laser beams
Growth
neurons
Neurons
Biochemistry
biochemistry
deflectors
Infrared lasers
controllability
Controllability
infrared lasers

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Carnegie, David J. ; Čižmár, Tomás ; Baumgartl, Jörg ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Automated laser guidance of neuronal growth cones using a spatial light modulator. In: Journal of Biophotonics. 2009 ; Vol. 2, No. 11. pp. 682-692.
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Automated laser guidance of neuronal growth cones using a spatial light modulator. / Carnegie, David J.; Čižmár, Tomás; Baumgartl, Jörg; Gunn-Moore, Frank J.; Dholakia, Kishan.

In: Journal of Biophotonics, Vol. 2, No. 11, 01.11.2009, p. 682-692.

Research output: Contribution to journalArticle

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