Automated laser guidance of neuronal growth cones using a spatial light modulator

David J. Carnegie; Tomás Čižmár; Jörg Baumgartl; Frank J. Gunn-Moore; Kishan Dholakia

doi:10.1002/jbio.200910043

Automated laser guidance of neuronal growth cones using a spatial light modulator

David J. Carnegie, Tomás Čižmár, Jörg Baumgartl, Frank J. Gunn-Moore, Kishan Dholakia

Department of Physics

Research output: Contribution to journal › Article

16 Citations (Scopus)

Abstract

The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.

Original language	English
Pages (from-to)	682-692
Number of pages	11
Journal	Journal of Biophotonics
Volume	2
Issue number	11
DOIs	https://doi.org/10.1002/jbio.200910043
Publication status	Published - 2009 Nov 1

All Science Journal Classification (ASJC) codes

Chemistry(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology(all)
Engineering(all)
Physics and Astronomy(all)

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10.1002/jbio.200910043

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Carnegie, D. J., Čižmár, T., Baumgartl, J., Gunn-Moore, F. J., & Dholakia, K. (2009). Automated laser guidance of neuronal growth cones using a spatial light modulator. Journal of Biophotonics, 2(11), 682-692. https://doi.org/10.1002/jbio.200910043

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