The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam . In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam . However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Physics and Astronomy(all)