Abstract
The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.
| Original language | English |
|---|---|
| Pages (from-to) | 682-692 |
| Number of pages | 11 |
| Journal | Journal of Biophotonics |
| Volume | 2 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2009 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Materials Science(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Engineering(all)
- Physics and Astronomy(all)
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Automated laser guidance of neuronal growth cones using a spatial light modulator. / Carnegie, David J.; Čižmár, Tomás; Baumgartl, Jörg; Gunn-Moore, Frank J.; Dholakia, Kishan.
In: Journal of Biophotonics, Vol. 2, No. 11, 01.11.2009, p. 682-692.Research output: Contribution to journal › Article
TY - JOUR
T1 - Automated laser guidance of neuronal growth cones using a spatial light modulator
AU - Carnegie, David J.
AU - Čižmár, Tomás
AU - Baumgartl, Jörg
AU - Gunn-Moore, Frank J.
AU - Dholakia, Kishan
PY - 2009/11/1
Y1 - 2009/11/1
N2 - The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.
AB - The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.
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U2 - 10.1002/jbio.200910043
DO - 10.1002/jbio.200910043
M3 - Article
C2 - 19705368
AN - SCOPUS:74049134736
VL - 2
SP - 682
EP - 692
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 11
ER -