Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser ablation is an established tool for studying cells' roles in development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of embryos/L1-stage Caenorhabditis elegans (C. elegans) and software for image processing and automation, allowing for high-throughput cell ablations. Large numbers of neuron-ablated and age-synchronized animals showed defects in chemotaxis avoidance behavior in a population assay.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages673-675
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

Fingerprint

Microsystems
Throughput
Networks (circuits)
Laser ablation
Ablation
Multiplexing
Microfluidics
Neurons
Assays
Animals
Image processing
Automation
Defects

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Chung, K., & Lu, H. (2009). Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 673-675). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.
Chung, Kwanghun ; Lu, Hang. / Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 673-675 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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Chung, K & Lu, H 2009, Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 673-675, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 09/11/1.

Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits. / Chung, Kwanghun; Lu, Hang.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 673-675 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chung K, Lu H. Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 673-675. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).