Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser ablation is an established tool for studying cells' roles in development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of embryos/L1-stage Caenorhabditis elegans (C. elegans) and software for image processing and automation, allowing for high-throughput cell ablations. Large numbers of neuron-ablated and age-synchronized animals showed defects in chemotaxis avoidance behavior in a population assay.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages673-675
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period09/11/109/11/5

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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