TY - GEN
T1 - Automated microsystem for high-throughput in vivo cell microsurgery to study neuronal circuits
AU - Chung, Kwanghun
AU - Lu, Hang
PY - 2009
Y1 - 2009
N2 - Laser ablation is an established tool for studying cells' roles in development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of embryos/L1-stage Caenorhabditis elegans (C. elegans) and software for image processing and automation, allowing for high-throughput cell ablations. Large numbers of neuron-ablated and age-synchronized animals showed defects in chemotaxis avoidance behavior in a population assay.
AB - Laser ablation is an established tool for studying cells' roles in development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of embryos/L1-stage Caenorhabditis elegans (C. elegans) and software for image processing and automation, allowing for high-throughput cell ablations. Large numbers of neuron-ablated and age-synchronized animals showed defects in chemotaxis avoidance behavior in a population assay.
UR - http://www.scopus.com/inward/record.url?scp=84901769267&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901769267&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901769267
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 673
EP - 675
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -