Autophagonizer, a novel synthetic small molecule, induces autophagic cell death

In Kwon Choi, Yoon Sun Cho, Hye Jin Jung, Ho Jeong Kwon

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Autophagy is an apoptosis-independent mechanism of cell death that protects the cell from environmental imbalances and infection by pathogens. We identified a novel small molecule, 2-(3-Benzyl-4-oxo-3,4,5,6,7,8-hexahydro-benzo[4,5]thieno[2,3-d]pyrimidin -2-ylsulfanylmethyl)-oxazole-4-carboxylic acid (2-pyrrolidin-1-yl-ethyl)-amide (referred as autophagonizer), using high-content cell-based screening and the autophagosome marker EGFP-LC3. Autophagonizer inhibited growth and induced cell death in the human tumor cell lines MCF7, HeLa, HCT116, A549, AGS, and HT1080 via a caspase-independent pathway. Conversion of cytosolic LC3-I to autophagosome-associated LC3-II was greatly enhanced by autophagonizer treatment. Transmission electron microscopy and acridine orange staining revealed increased autophagy in the cytoplasm of autophagonizer-treated cells. In conclusion, autophagonizer is a novel autophagy inducer with unique structure, which induces autophagic cell death in the human tumor cell lines.

Original languageEnglish
Pages (from-to)849-854
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume393
Issue number4
DOIs
Publication statusPublished - 2010 Mar 19

Fingerprint

Autophagy
Cell death
Molecules
Cells
Tumor Cell Line
Tumors
Cell Death
Oxazoles
Acridine Orange
Pathogens
Caspases
Carboxylic Acids
Transmission Electron Microscopy
Amides
Screening
Cytoplasm
2-(3-benzyl-4-oxo-3,4,5,6,7,8-hexahydrobenzo(4,5)thieno(2,3-d)pyrimidin-2-ylsulfanylmethyl)-oxazole-4-carboxylic acid (2-pyrrolidin-1-yl-ethyl)amide
Apoptosis
Staining and Labeling
Transmission electron microscopy

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Autophagy is an apoptosis-independent mechanism of cell death that protects the cell from environmental imbalances and infection by pathogens. We identified a novel small molecule, 2-(3-Benzyl-4-oxo-3,4,5,6,7,8-hexahydro-benzo[4,5]thieno[2,3-d]pyrimidin -2-ylsulfanylmethyl)-oxazole-4-carboxylic acid (2-pyrrolidin-1-yl-ethyl)-amide (referred as autophagonizer), using high-content cell-based screening and the autophagosome marker EGFP-LC3. Autophagonizer inhibited growth and induced cell death in the human tumor cell lines MCF7, HeLa, HCT116, A549, AGS, and HT1080 via a caspase-independent pathway. Conversion of cytosolic LC3-I to autophagosome-associated LC3-II was greatly enhanced by autophagonizer treatment. Transmission electron microscopy and acridine orange staining revealed increased autophagy in the cytoplasm of autophagonizer-treated cells. In conclusion, autophagonizer is a novel autophagy inducer with unique structure, which induces autophagic cell death in the human tumor cell lines.",
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Autophagonizer, a novel synthetic small molecule, induces autophagic cell death. / Choi, In Kwon; Cho, Yoon Sun; Jung, Hye Jin; Kwon, Ho Jeong.

In: Biochemical and Biophysical Research Communications, Vol. 393, No. 4, 19.03.2010, p. 849-854.

Research output: Contribution to journalArticle

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