Autophagy induction can regulate skin pigmentation by causing melanosome degradation in keratinocytes and melanocytes

Ji Young Kim, Jihee Kim, Yuri Ahn, Eun Jung Lee, Shinwon Hwang, Abdurrahman Almurayshid, Keedon Park, Hwa Jee Chung, Heung Jae Kim, Si Hyung Lee, Myung Shik Lee, Sang Ho Oh

Research output: Contribution to journalArticle

Abstract

Autophagy regulates cellular turnover by disassembling unnecessary or dysfunctional constituents. Recent studies demonstrated that autophagy and its regulators play a wide variety of roles in melanocyte biology. Activation of autophagy is known to induce melanogenesis and regulate melanosome biogenesis in melanocytes. Also, autophagy induction was reported to regulate physiologic skin color via melanosome degradation, although the downstream effectors are not yet clarified. To determine the role of autophagy as a melanosome degradation machinery, we administered several autophagy inducers in human keratinocytes and melanocytes. Our results showed that the synthetic autophagy inducer PTPD-12 stimulated autophagic flux in human melanocytes and in keratinocytes containing transferred melanosomes. Increased autophagic flux led to melanosome degradation without affecting the expression of MITF. Furthermore, the color of cell pellets of both melanocytes and keratinocytes was visibly lightened. Inhibition of autophagic flux by chloroquine resulted in marked attenuation of PTPD-12-induced melanosome degradation, whereas the expression of melanogenesis pathway genes and proteins remained unaffected. Taken together, our results suggest that the modulation of autophagy can contribute to the regulation of melanocyte biology and skin pigmentation.

Original languageEnglish
JournalPigment Cell and Melanoma Research
DOIs
Publication statusAccepted/In press - 2019 Jan 1

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Skin Pigmentation
Melanosomes
Melanocytes
Autophagy
Keratinocytes
Skin
Degradation
Fluxes
Color
Chloroquine
Machinery
Chemical activation
Modulation
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Dermatology

Cite this

Kim, Ji Young ; Kim, Jihee ; Ahn, Yuri ; Lee, Eun Jung ; Hwang, Shinwon ; Almurayshid, Abdurrahman ; Park, Keedon ; Chung, Hwa Jee ; Kim, Heung Jae ; Lee, Si Hyung ; Lee, Myung Shik ; Oh, Sang Ho. / Autophagy induction can regulate skin pigmentation by causing melanosome degradation in keratinocytes and melanocytes. In: Pigment Cell and Melanoma Research. 2019.
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abstract = "Autophagy regulates cellular turnover by disassembling unnecessary or dysfunctional constituents. Recent studies demonstrated that autophagy and its regulators play a wide variety of roles in melanocyte biology. Activation of autophagy is known to induce melanogenesis and regulate melanosome biogenesis in melanocytes. Also, autophagy induction was reported to regulate physiologic skin color via melanosome degradation, although the downstream effectors are not yet clarified. To determine the role of autophagy as a melanosome degradation machinery, we administered several autophagy inducers in human keratinocytes and melanocytes. Our results showed that the synthetic autophagy inducer PTPD-12 stimulated autophagic flux in human melanocytes and in keratinocytes containing transferred melanosomes. Increased autophagic flux led to melanosome degradation without affecting the expression of MITF. Furthermore, the color of cell pellets of both melanocytes and keratinocytes was visibly lightened. Inhibition of autophagic flux by chloroquine resulted in marked attenuation of PTPD-12-induced melanosome degradation, whereas the expression of melanogenesis pathway genes and proteins remained unaffected. Taken together, our results suggest that the modulation of autophagy can contribute to the regulation of melanocyte biology and skin pigmentation.",
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Autophagy induction can regulate skin pigmentation by causing melanosome degradation in keratinocytes and melanocytes. / Kim, Ji Young; Kim, Jihee; Ahn, Yuri; Lee, Eun Jung; Hwang, Shinwon; Almurayshid, Abdurrahman; Park, Keedon; Chung, Hwa Jee; Kim, Heung Jae; Lee, Si Hyung; Lee, Myung Shik; Oh, Sang Ho.

In: Pigment Cell and Melanoma Research, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Hwang, Shinwon

AU - Almurayshid, Abdurrahman

AU - Park, Keedon

AU - Chung, Hwa Jee

AU - Kim, Heung Jae

AU - Lee, Si Hyung

AU - Lee, Myung Shik

AU - Oh, Sang Ho

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