Auxin induces three genes encoding 1-aminocyclopropane-1-carboxylate synthase in mung bean hypocotyls

Taek Kim Woo Taek Kim, A. Campbell, T. Moriguchi, Chul Yi Ho Chul Yi, Fa Yang Shang Fa Yang

Research output: Contribution to journalArticle

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Abstract

By screening a cDNA library of auxin-treated mung bean (Vigna radiata L.) hypocotyls, we have isolated two full-length cDNA clones, pVR-ACS6 and pVR-ACS7, for 1-aminocyclopropane-1-carboxylate (ACC) synthase, the rate-limiting enzyme in the ethylene biosynthetic pathway. While pVR-ACS6 corresponds to the previously identified PCR fragment pMBA1, pVR-ACS7 is a new cDNA clone. pVR-ACS6 is 1867bp long encoding 472 amino acids (Mr = 53.6 kDa), and pVR-ACS7 is a 1840 bp clone encoding 468 amino acids (Mr = 53.1 kDa). The coding regions of pVR-ACS6 and pVR-ACS7 share 81 and 88% identity at nucleotide and amino acid levels, respectively. Genomic Southern blot analyses suggest the existence of only one copy of ACS6 and ACS7 genes in the mung bean genome. Previously, it was reported that mung bean ACC synthase cDNA clone (pAIM-1) representing ACS1 gene was expressed following auxin treatment. Northern blot analyses were carried out to compare the magnitudes and induction kinetics of the expression of the three genes, ACS1, ACS6 and ACS7 whose expressions are induced by auxin (100 μM IAA) in hypocotyls. Although all three genes are expressed in response to auxin treatment, the level of ACS1 transcript is lower than those of ACS6 and ACS7 transcripts during the entire period of auxin treatment. ACS6 and ACS7 are specifically induced by auxin, while ACS1 is induced by cycloheximide, and to lesser extent by excision and auxin treatment. A comparison of deduced amino acid sequences among auxin-induced ACC synthases reveals that these enzymes share a high degree of homology (65-75%) to VR-ACS6 and VR-ACS7 polypeptides, but only about 50% to VR-ACS1 polypeptide. These results indicate that ACC synthase is encoded by a multigene family and expression of these genes is differentially regulated by auxin in mung bean hypocotyl tissue.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalJournal of Plant Physiology
Volume150
Issue number1-2
Publication statusPublished - 1997 Jan 1

Fingerprint

1-aminocyclopropanecarboxylate synthase
1-aminocyclopropane-1-carboxylate synthase
Hypocotyl
Indoleacetic Acids
mung beans
hypocotyls
auxins
Genes
genes
Clone Cells
clones
Complementary DNA
Amino Acids
amino acids
polypeptides
Gene Expression
Vigna
Peptides
Vigna radiata
Biosynthetic Pathways

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Woo Taek Kim, T. K., Campbell, A., Moriguchi, T., Ho Chul Yi, C. Y., & Shang Fa Yang, F. Y. (1997). Auxin induces three genes encoding 1-aminocyclopropane-1-carboxylate synthase in mung bean hypocotyls. Journal of Plant Physiology, 150(1-2), 77-84.
Woo Taek Kim, Taek Kim ; Campbell, A. ; Moriguchi, T. ; Ho Chul Yi, Chul Yi ; Shang Fa Yang, Fa Yang. / Auxin induces three genes encoding 1-aminocyclopropane-1-carboxylate synthase in mung bean hypocotyls. In: Journal of Plant Physiology. 1997 ; Vol. 150, No. 1-2. pp. 77-84.
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abstract = "By screening a cDNA library of auxin-treated mung bean (Vigna radiata L.) hypocotyls, we have isolated two full-length cDNA clones, pVR-ACS6 and pVR-ACS7, for 1-aminocyclopropane-1-carboxylate (ACC) synthase, the rate-limiting enzyme in the ethylene biosynthetic pathway. While pVR-ACS6 corresponds to the previously identified PCR fragment pMBA1, pVR-ACS7 is a new cDNA clone. pVR-ACS6 is 1867bp long encoding 472 amino acids (Mr = 53.6 kDa), and pVR-ACS7 is a 1840 bp clone encoding 468 amino acids (Mr = 53.1 kDa). The coding regions of pVR-ACS6 and pVR-ACS7 share 81 and 88{\%} identity at nucleotide and amino acid levels, respectively. Genomic Southern blot analyses suggest the existence of only one copy of ACS6 and ACS7 genes in the mung bean genome. Previously, it was reported that mung bean ACC synthase cDNA clone (pAIM-1) representing ACS1 gene was expressed following auxin treatment. Northern blot analyses were carried out to compare the magnitudes and induction kinetics of the expression of the three genes, ACS1, ACS6 and ACS7 whose expressions are induced by auxin (100 μM IAA) in hypocotyls. Although all three genes are expressed in response to auxin treatment, the level of ACS1 transcript is lower than those of ACS6 and ACS7 transcripts during the entire period of auxin treatment. ACS6 and ACS7 are specifically induced by auxin, while ACS1 is induced by cycloheximide, and to lesser extent by excision and auxin treatment. A comparison of deduced amino acid sequences among auxin-induced ACC synthases reveals that these enzymes share a high degree of homology (65-75{\%}) to VR-ACS6 and VR-ACS7 polypeptides, but only about 50{\%} to VR-ACS1 polypeptide. These results indicate that ACC synthase is encoded by a multigene family and expression of these genes is differentially regulated by auxin in mung bean hypocotyl tissue.",
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Woo Taek Kim, TK, Campbell, A, Moriguchi, T, Ho Chul Yi, CY & Shang Fa Yang, FY 1997, 'Auxin induces three genes encoding 1-aminocyclopropane-1-carboxylate synthase in mung bean hypocotyls', Journal of Plant Physiology, vol. 150, no. 1-2, pp. 77-84.

Auxin induces three genes encoding 1-aminocyclopropane-1-carboxylate synthase in mung bean hypocotyls. / Woo Taek Kim, Taek Kim; Campbell, A.; Moriguchi, T.; Ho Chul Yi, Chul Yi; Shang Fa Yang, Fa Yang.

In: Journal of Plant Physiology, Vol. 150, No. 1-2, 01.01.1997, p. 77-84.

Research output: Contribution to journalArticle

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AU - Woo Taek Kim, Taek Kim

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AB - By screening a cDNA library of auxin-treated mung bean (Vigna radiata L.) hypocotyls, we have isolated two full-length cDNA clones, pVR-ACS6 and pVR-ACS7, for 1-aminocyclopropane-1-carboxylate (ACC) synthase, the rate-limiting enzyme in the ethylene biosynthetic pathway. While pVR-ACS6 corresponds to the previously identified PCR fragment pMBA1, pVR-ACS7 is a new cDNA clone. pVR-ACS6 is 1867bp long encoding 472 amino acids (Mr = 53.6 kDa), and pVR-ACS7 is a 1840 bp clone encoding 468 amino acids (Mr = 53.1 kDa). The coding regions of pVR-ACS6 and pVR-ACS7 share 81 and 88% identity at nucleotide and amino acid levels, respectively. Genomic Southern blot analyses suggest the existence of only one copy of ACS6 and ACS7 genes in the mung bean genome. Previously, it was reported that mung bean ACC synthase cDNA clone (pAIM-1) representing ACS1 gene was expressed following auxin treatment. Northern blot analyses were carried out to compare the magnitudes and induction kinetics of the expression of the three genes, ACS1, ACS6 and ACS7 whose expressions are induced by auxin (100 μM IAA) in hypocotyls. Although all three genes are expressed in response to auxin treatment, the level of ACS1 transcript is lower than those of ACS6 and ACS7 transcripts during the entire period of auxin treatment. ACS6 and ACS7 are specifically induced by auxin, while ACS1 is induced by cycloheximide, and to lesser extent by excision and auxin treatment. A comparison of deduced amino acid sequences among auxin-induced ACC synthases reveals that these enzymes share a high degree of homology (65-75%) to VR-ACS6 and VR-ACS7 polypeptides, but only about 50% to VR-ACS1 polypeptide. These results indicate that ACC synthase is encoded by a multigene family and expression of these genes is differentially regulated by auxin in mung bean hypocotyl tissue.

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