Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea.

S. J. Park, Taisoon Yong, H. W. Yang, D. H. Lee, Kyungwon Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Inoculating of human fecal cysts to suckling Mongolian gerbils, two Giardia lamblia isolates, K1 and K2, were established as axenic cultures. Using this in vitro culture, both isolates were characterized as a "medium-rate grower" upon its growth pattern. These two Giardia isolates were grouped by using two genetic analysis. With genetic analysis of SSU-rDNA sequences, both K1 and K2 were found as members of Hopkins' group 1, despite some nucleotide differences noticed in K2 (5 differences/292 bases). The other genetic study used PCR-RFLP of the tim (triose phosphate isomerase) gene. Both of K1 and K2 were found to belong to Nash's group 2. Our results suggest that Nash's group 2 can not be a separate group, but a part of Hopkins' group 1.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalThe Korean journal of parasitology
Volume37
Issue number2
DOIs
Publication statusPublished - 1999 Jan 1

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Axenic Culture
Triose-Phosphate Isomerase
Giardia
Giardia lamblia
Gerbillinae
Korea
Ribosomal DNA
Restriction Fragment Length Polymorphisms
Cysts
Nucleotides
Polymerase Chain Reaction
Growth
Genes
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

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Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea. / Park, S. J.; Yong, Taisoon; Yang, H. W.; Lee, D. H.; Lee, Kyungwon.

In: The Korean journal of parasitology, Vol. 37, No. 2, 01.01.1999, p. 121-125.

Research output: Contribution to journalArticle

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