Axial Scanning Metal-Induced Energy Transfer Microscopy for Extended Range Nanometer-Sectioning Cell Imaging

Wonsang Hwang, Dongeun Kim, Dugyoung Kim

Research output: Contribution to journalArticlepeer-review


Nanometer-sectioning optical microscopy has become an indispensable tool in membrane-related biomedical studies. Finally, many nanometer-sectioning imaging schemes, such as variable-angle total internal reflection fluorescence microscopy, metal-induced energy transfer (MIET) imaging, and supercritical-angle fluorescence microscopy have been introduced. However, these methods can measure a single layer of molecules, and the measurement ranges are below 100 nm, which is not large enough to cover the thickness of lamellipodium. This paper proposes an optical imaging scheme that can identify the axial locations of two layers of molecules with an extended measurement range and a nanometer-scale precision by using MIET, axial focal plane scanning, and biexponential analysis in fluorescence lifetime imaging microscopy. The feasibility of the proposed method is demonstrated by measuring an artificial sample of a known structure and the lamellipodium of a human aortic endothelial cell whose thickness ranges from 100 to 450 nm with 18.3 nm precision.

Original languageEnglish
Article number2105497
Issue number7
Publication statusPublished - 2022 Feb 17

Bibliographical note

Funding Information:
This work was supported by The National Research Foundation of Korea (NRF) through the Basic Science Program (No. 2021R1A2C2009090), Korea Institute for Advancement of Technology (KIAT) and the Swiss Innovation Agency (Innosuisse) through South Korea‐Switzerland Joint Innovation Project (No. P0011925).

Publisher Copyright:
© 2021 Wiley-VCH GmbH

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Chemistry(all)
  • Biomaterials
  • Materials Science(all)


Dive into the research topics of 'Axial Scanning Metal-Induced Energy Transfer Microscopy for Extended Range Nanometer-Sectioning Cell Imaging'. Together they form a unique fingerprint.

Cite this