B lymphocyte deficiency in IgH-transgenic rabbits

Paul J. Jasper, Ki Jong Rhee, Susan L. Kalis, Periannan Sethupathi, Pi Chen Yam, Shi Kang Zhai, Katherine L. Knight

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We developed IgH-transgenic rabbits carrying a productive VDJ-Cμ Tg and found the rabbits were B cell-deficient, with a 50-100% reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ-Cμ Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg μ-chain paired with VpreB and λ5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous μ-chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle-associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle-associated epithelium, possibly through contact with intestinal microbiota. These IgH-Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT.

Original languageEnglish
Pages (from-to)2290-2299
Number of pages10
JournalEuropean Journal of Immunology
Volume37
Issue number8
DOIs
Publication statusPublished - 2007 Aug 1

Fingerprint

B-Lymphocytes
Rabbits
Bone Marrow
Epithelium
Immunoprecipitation
Immunoglobulin M
Immunoglobulin G
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Jasper, P. J., Rhee, K. J., Kalis, S. L., Sethupathi, P., Yam, P. C., Zhai, S. K., & Knight, K. L. (2007). B lymphocyte deficiency in IgH-transgenic rabbits. European Journal of Immunology, 37(8), 2290-2299. https://doi.org/10.1002/eji.200737191
Jasper, Paul J. ; Rhee, Ki Jong ; Kalis, Susan L. ; Sethupathi, Periannan ; Yam, Pi Chen ; Zhai, Shi Kang ; Knight, Katherine L. / B lymphocyte deficiency in IgH-transgenic rabbits. In: European Journal of Immunology. 2007 ; Vol. 37, No. 8. pp. 2290-2299.
@article{c1170bb3f1dd413ba20fd4169b17b41e,
title = "B lymphocyte deficiency in IgH-transgenic rabbits",
abstract = "We developed IgH-transgenic rabbits carrying a productive VDJ-Cμ Tg and found the rabbits were B cell-deficient, with a 50-100{\%} reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ-Cμ Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg μ-chain paired with VpreB and λ5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous μ-chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle-associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle-associated epithelium, possibly through contact with intestinal microbiota. These IgH-Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT.",
author = "Jasper, {Paul J.} and Rhee, {Ki Jong} and Kalis, {Susan L.} and Periannan Sethupathi and Yam, {Pi Chen} and Zhai, {Shi Kang} and Knight, {Katherine L.}",
year = "2007",
month = "8",
day = "1",
doi = "10.1002/eji.200737191",
language = "English",
volume = "37",
pages = "2290--2299",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "8",

}

Jasper, PJ, Rhee, KJ, Kalis, SL, Sethupathi, P, Yam, PC, Zhai, SK & Knight, KL 2007, 'B lymphocyte deficiency in IgH-transgenic rabbits', European Journal of Immunology, vol. 37, no. 8, pp. 2290-2299. https://doi.org/10.1002/eji.200737191

B lymphocyte deficiency in IgH-transgenic rabbits. / Jasper, Paul J.; Rhee, Ki Jong; Kalis, Susan L.; Sethupathi, Periannan; Yam, Pi Chen; Zhai, Shi Kang; Knight, Katherine L.

In: European Journal of Immunology, Vol. 37, No. 8, 01.08.2007, p. 2290-2299.

Research output: Contribution to journalArticle

TY - JOUR

T1 - B lymphocyte deficiency in IgH-transgenic rabbits

AU - Jasper, Paul J.

AU - Rhee, Ki Jong

AU - Kalis, Susan L.

AU - Sethupathi, Periannan

AU - Yam, Pi Chen

AU - Zhai, Shi Kang

AU - Knight, Katherine L.

PY - 2007/8/1

Y1 - 2007/8/1

N2 - We developed IgH-transgenic rabbits carrying a productive VDJ-Cμ Tg and found the rabbits were B cell-deficient, with a 50-100% reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ-Cμ Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg μ-chain paired with VpreB and λ5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous μ-chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle-associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle-associated epithelium, possibly through contact with intestinal microbiota. These IgH-Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT.

AB - We developed IgH-transgenic rabbits carrying a productive VDJ-Cμ Tg and found the rabbits were B cell-deficient, with a 50-100% reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ-Cμ Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg μ-chain paired with VpreB and λ5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous μ-chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle-associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle-associated epithelium, possibly through contact with intestinal microbiota. These IgH-Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT.

UR - http://www.scopus.com/inward/record.url?scp=34547859641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547859641&partnerID=8YFLogxK

U2 - 10.1002/eji.200737191

DO - 10.1002/eji.200737191

M3 - Article

C2 - 17628859

AN - SCOPUS:34547859641

VL - 37

SP - 2290

EP - 2299

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 8

ER -

Jasper PJ, Rhee KJ, Kalis SL, Sethupathi P, Yam PC, Zhai SK et al. B lymphocyte deficiency in IgH-transgenic rabbits. European Journal of Immunology. 2007 Aug 1;37(8):2290-2299. https://doi.org/10.1002/eji.200737191