B lymphocyte deficiency in IgH-transgenic rabbits

Paul J. Jasper, Ki Jong Rhee, Susan L. Kalis, Periannan Sethupathi, Pi Chen Yam, Shi Kang Zhai, Katherine L. Knight

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Abstract

We developed IgH-transgenic rabbits carrying a productive VDJ-Cμ Tg and found the rabbits were B cell-deficient, with a 50-100% reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ-Cμ Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg μ-chain paired with VpreB and λ5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous μ-chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle-associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle-associated epithelium, possibly through contact with intestinal microbiota. These IgH-Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT.

Original languageEnglish
Pages (from-to)2290-2299
Number of pages10
JournalEuropean Journal of Immunology
Volume37
Issue number8
DOIs
Publication statusPublished - 2007 Aug 1

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Jasper, P. J., Rhee, K. J., Kalis, S. L., Sethupathi, P., Yam, P. C., Zhai, S. K., & Knight, K. L. (2007). B lymphocyte deficiency in IgH-transgenic rabbits. European Journal of Immunology, 37(8), 2290-2299. https://doi.org/10.1002/eji.200737191