Abstract
An atypical orphan response regulator protein, HP1043 (HP-RR) in Helicobacter pylori, is proven to be essential for cell growth and does not require the well known phosphorelay scheme. HP-RR was identified as a symmetric dimer with two functional domains, an N-terminal regulatory domain (HP-RR r) and a C-terminal effector domain (HP-RRe). HP-RR is a new class of response regulator, as a phosphorylation-independent regulator. Previously, we have presented a detailed three-dimensional structure of HP-RR using NMR spectroscopy and X-ray crystallography. In this study, in order to understand the functional importance of flexibilities in HP-RRr and HP-RRe, T1, T2, heteronuclear NOE experiments have been performed and backbone dynamics of HP-RRr and HP-RR e were investigated. HP-RRr is a symmetric dimer and the interface region, α4-β5-α5 of dimer, showed high rigidity (high S 2 values). Site of rearrangements associated with phosphorylation of HP-RRr (Ser75: R ex = 3.382, Ile 95: R ex = 5.228) showed slow chemical exchanges. HP-RRe is composed of three α-helices flanked on two sides by anti-parallel β-sheets. Low order parameters as well as conformational exchanges in the centers of loop regions known as the DNA binding site and transcription site of HP-RRe suggested that flexibility of HP-RR e is essential for interaction with DNA. In conclusion, backbone dynamics information for HP-RR implies that structural flexibilities in HP-RRr are necessary for the phosphorylation site and the dynamic nature of HP-RRe is essential for the regulation of interaction between protein and DNA.
Original language | English |
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Pages (from-to) | 158-165 |
Number of pages | 8 |
Journal | Molecules and cells |
Volume | 35 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2013 Feb |
Bibliographical note
Funding Information:This work was supported by grants from the Basic Science Research Program (2011-0022873, Y. Kim), Priority Research Centers Program (2012-0006686, Y. Kim) through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology, and World Class University (WCU) program (R33-2009-000-10123-0, W. Lee).
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology