Bactericidal effect through non-uptake pathway with photofunctional silicon polymer that generates reactive oxygen species

Kang Kyun Wang, Seung Jin Jung, Jeong Wook Hwang, Bong Jin Kim, Da Hee Kim, Il Kwon Bae, Seok Hoon Jeong, Yong Rok Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We report bactericidal effect of photosensitizer (H2TPP: 5,10,15,20-tetraphenyl-21H,23H-porphyrin) through non-uptake pathway and efficacy of the photofunctional silicon polymer to the decomposition of the formed biofilm and the suppression of the biofilm formation. The photofunctional silicon polymer (PSP), which is the silicon polymer embedded with a photosensitizer, is fabricated by a simple solvent swell-encapsulation-shrink method. Reactive oxygen generation from PSP was confirmed by using the decomposition reaction of 1,3-diphnyl-isobenzofuran (DPBF). Also, singlet oxygen generation which is one of the reactive oxygen species (ROS) from PSP is directly confirmed with time and wavelength resolved singlet oxygen phosphorescence spectroscopy. For the influence study of ROS under the non-uptake condition of photosensitizer (PS to bacteria), photodynamic inactivation (PDI) effect of PSP is evaluated for Gram-positive, Gram-negative bacteria, and fungi. Those microorganisms were inactivated by PSP within 1 h under the given power of laser light (63.7 mW/cm2). Among the bacteria, especially, Staphylococcus aureus as the Gram-positive bacteria were completely disinfected under the given experimental condition. Furthermore, PSP successfully demonstrates the decomposition of the formed biofilm and the suppression of the biofilm formation with green light emitting diode (GLED, 3.5 mW/cm2, λmax = 517 nm, FWHM = 37 nm), which shows the practical application possibility of bactericidal material.

Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalJournal of Photochemistry and Photobiology A: Chemistry
Volume315
DOIs
Publication statusPublished - 2016 Jan 15

Fingerprint

silicon polymers
Silicon
Reactive Oxygen Species
Polymers
biofilms
Oxygen
Biofilms
oxygen
bacteria
Photosensitizing Agents
Photosensitizers
Bacteria
Singlet Oxygen
Decomposition
decomposition
retarding
Phosphorescence
staphylococcus
fungi
Porphyrins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Physics and Astronomy(all)

Cite this

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title = "Bactericidal effect through non-uptake pathway with photofunctional silicon polymer that generates reactive oxygen species",
abstract = "We report bactericidal effect of photosensitizer (H2TPP: 5,10,15,20-tetraphenyl-21H,23H-porphyrin) through non-uptake pathway and efficacy of the photofunctional silicon polymer to the decomposition of the formed biofilm and the suppression of the biofilm formation. The photofunctional silicon polymer (PSP), which is the silicon polymer embedded with a photosensitizer, is fabricated by a simple solvent swell-encapsulation-shrink method. Reactive oxygen generation from PSP was confirmed by using the decomposition reaction of 1,3-diphnyl-isobenzofuran (DPBF). Also, singlet oxygen generation which is one of the reactive oxygen species (ROS) from PSP is directly confirmed with time and wavelength resolved singlet oxygen phosphorescence spectroscopy. For the influence study of ROS under the non-uptake condition of photosensitizer (PS to bacteria), photodynamic inactivation (PDI) effect of PSP is evaluated for Gram-positive, Gram-negative bacteria, and fungi. Those microorganisms were inactivated by PSP within 1 h under the given power of laser light (63.7 mW/cm2). Among the bacteria, especially, Staphylococcus aureus as the Gram-positive bacteria were completely disinfected under the given experimental condition. Furthermore, PSP successfully demonstrates the decomposition of the formed biofilm and the suppression of the biofilm formation with green light emitting diode (GLED, 3.5 mW/cm2, λmax = 517 nm, FWHM = 37 nm), which shows the practical application possibility of bactericidal material.",
author = "Wang, {Kang Kyun} and Jung, {Seung Jin} and Hwang, {Jeong Wook} and Kim, {Bong Jin} and Kim, {Da Hee} and Bae, {Il Kwon} and Jeong, {Seok Hoon} and Kim, {Yong Rok}",
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Bactericidal effect through non-uptake pathway with photofunctional silicon polymer that generates reactive oxygen species. / Wang, Kang Kyun; Jung, Seung Jin; Hwang, Jeong Wook; Kim, Bong Jin; Kim, Da Hee; Bae, Il Kwon; Jeong, Seok Hoon; Kim, Yong Rok.

In: Journal of Photochemistry and Photobiology A: Chemistry, Vol. 315, 15.01.2016, p. 52-58.

Research output: Contribution to journalArticle

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T1 - Bactericidal effect through non-uptake pathway with photofunctional silicon polymer that generates reactive oxygen species

AU - Wang, Kang Kyun

AU - Jung, Seung Jin

AU - Hwang, Jeong Wook

AU - Kim, Bong Jin

AU - Kim, Da Hee

AU - Bae, Il Kwon

AU - Jeong, Seok Hoon

AU - Kim, Yong Rok

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N2 - We report bactericidal effect of photosensitizer (H2TPP: 5,10,15,20-tetraphenyl-21H,23H-porphyrin) through non-uptake pathway and efficacy of the photofunctional silicon polymer to the decomposition of the formed biofilm and the suppression of the biofilm formation. The photofunctional silicon polymer (PSP), which is the silicon polymer embedded with a photosensitizer, is fabricated by a simple solvent swell-encapsulation-shrink method. Reactive oxygen generation from PSP was confirmed by using the decomposition reaction of 1,3-diphnyl-isobenzofuran (DPBF). Also, singlet oxygen generation which is one of the reactive oxygen species (ROS) from PSP is directly confirmed with time and wavelength resolved singlet oxygen phosphorescence spectroscopy. For the influence study of ROS under the non-uptake condition of photosensitizer (PS to bacteria), photodynamic inactivation (PDI) effect of PSP is evaluated for Gram-positive, Gram-negative bacteria, and fungi. Those microorganisms were inactivated by PSP within 1 h under the given power of laser light (63.7 mW/cm2). Among the bacteria, especially, Staphylococcus aureus as the Gram-positive bacteria were completely disinfected under the given experimental condition. Furthermore, PSP successfully demonstrates the decomposition of the formed biofilm and the suppression of the biofilm formation with green light emitting diode (GLED, 3.5 mW/cm2, λmax = 517 nm, FWHM = 37 nm), which shows the practical application possibility of bactericidal material.

AB - We report bactericidal effect of photosensitizer (H2TPP: 5,10,15,20-tetraphenyl-21H,23H-porphyrin) through non-uptake pathway and efficacy of the photofunctional silicon polymer to the decomposition of the formed biofilm and the suppression of the biofilm formation. The photofunctional silicon polymer (PSP), which is the silicon polymer embedded with a photosensitizer, is fabricated by a simple solvent swell-encapsulation-shrink method. Reactive oxygen generation from PSP was confirmed by using the decomposition reaction of 1,3-diphnyl-isobenzofuran (DPBF). Also, singlet oxygen generation which is one of the reactive oxygen species (ROS) from PSP is directly confirmed with time and wavelength resolved singlet oxygen phosphorescence spectroscopy. For the influence study of ROS under the non-uptake condition of photosensitizer (PS to bacteria), photodynamic inactivation (PDI) effect of PSP is evaluated for Gram-positive, Gram-negative bacteria, and fungi. Those microorganisms were inactivated by PSP within 1 h under the given power of laser light (63.7 mW/cm2). Among the bacteria, especially, Staphylococcus aureus as the Gram-positive bacteria were completely disinfected under the given experimental condition. Furthermore, PSP successfully demonstrates the decomposition of the formed biofilm and the suppression of the biofilm formation with green light emitting diode (GLED, 3.5 mW/cm2, λmax = 517 nm, FWHM = 37 nm), which shows the practical application possibility of bactericidal material.

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