BAK7 displays unequal genetic redundancy with BAK1 in brassinosteroid signaling and early senescence in arabidopsis

Yu Jeong Jeong, Yun Shang, Beg Hab Kim, Sun Young Kim, Jae Hyo Song, June Seung Lee, Myeong Min Lee, Jianming Li, Kyoung Hee Nam

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

BRI1-Associated kinase1 (BAK1), a five leucine-rich-repeat containing receptor-like serine/threonine kinase, has been shown to have dual functions: mediating brassinosteroid (BR) signaling and acting in the BR-independent plant defense response. Sequence analysis has revealed that BAK1 has two homologs, BAK7 and BAK8. Because BAK8 deviates from the canonical RD kinase motif, we focused on the functional analysis of BAK7. The expression pattern and tissues in which BAK7 appeared partially overlapped with those observed for BAK1. Expression levels of BAK7 increased in the bak1 mutant. Overexpression of BAK7 rescued the bri1 mutant phenotype, indicating that BAK7 can compensate for BAK1 in BR-mediated processes, especially in the absence of BAK1. However, root and hypocotyl elongation patterns of transgenic plants overexpressing BAK1 or BAK7 appeared to be different from the patterns observed in a BRI1 overexpressor. Furthermore, the sensitivity of transgenic plants overexpressing BAK7 to brassinazole, a biosynthetic inhibitor of brassinolide (BL), did not change compared to that of wild-type plants. In addition, we generated transgenic plants expressing BAK7 RNA interference constructs and found severe growth retardation and early senescence in these lines. Taken together, these results suggest that BAK7 is a component of the BR signaling pathway, with varying degrees of genetic redundancy with BAK1, and that it affects plant growth via BL-independent pathways in vivo.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalMolecules and cells
Volume29
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

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Brassinosteroids
Arabidopsis
Genetically Modified Plants
Hypocotyl
Protein-Serine-Threonine Kinases
Growth
RNA Interference
Leucine
Sequence Analysis
Phosphotransferases
Phenotype

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Jeong, Y. J., Shang, Y., Kim, B. H., Kim, S. Y., Song, J. H., Lee, J. S., ... Nam, K. H. (2010). BAK7 displays unequal genetic redundancy with BAK1 in brassinosteroid signaling and early senescence in arabidopsis. Molecules and cells, 29(3), 259-266. https://doi.org/10.1007/s10059-010-0024-0
Jeong, Yu Jeong ; Shang, Yun ; Kim, Beg Hab ; Kim, Sun Young ; Song, Jae Hyo ; Lee, June Seung ; Lee, Myeong Min ; Li, Jianming ; Nam, Kyoung Hee. / BAK7 displays unequal genetic redundancy with BAK1 in brassinosteroid signaling and early senescence in arabidopsis. In: Molecules and cells. 2010 ; Vol. 29, No. 3. pp. 259-266.
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abstract = "BRI1-Associated kinase1 (BAK1), a five leucine-rich-repeat containing receptor-like serine/threonine kinase, has been shown to have dual functions: mediating brassinosteroid (BR) signaling and acting in the BR-independent plant defense response. Sequence analysis has revealed that BAK1 has two homologs, BAK7 and BAK8. Because BAK8 deviates from the canonical RD kinase motif, we focused on the functional analysis of BAK7. The expression pattern and tissues in which BAK7 appeared partially overlapped with those observed for BAK1. Expression levels of BAK7 increased in the bak1 mutant. Overexpression of BAK7 rescued the bri1 mutant phenotype, indicating that BAK7 can compensate for BAK1 in BR-mediated processes, especially in the absence of BAK1. However, root and hypocotyl elongation patterns of transgenic plants overexpressing BAK1 or BAK7 appeared to be different from the patterns observed in a BRI1 overexpressor. Furthermore, the sensitivity of transgenic plants overexpressing BAK7 to brassinazole, a biosynthetic inhibitor of brassinolide (BL), did not change compared to that of wild-type plants. In addition, we generated transgenic plants expressing BAK7 RNA interference constructs and found severe growth retardation and early senescence in these lines. Taken together, these results suggest that BAK7 is a component of the BR signaling pathway, with varying degrees of genetic redundancy with BAK1, and that it affects plant growth via BL-independent pathways in vivo.",
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BAK7 displays unequal genetic redundancy with BAK1 in brassinosteroid signaling and early senescence in arabidopsis. / Jeong, Yu Jeong; Shang, Yun; Kim, Beg Hab; Kim, Sun Young; Song, Jae Hyo; Lee, June Seung; Lee, Myeong Min; Li, Jianming; Nam, Kyoung Hee.

In: Molecules and cells, Vol. 29, No. 3, 01.03.2010, p. 259-266.

Research output: Contribution to journalArticle

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AU - Jeong, Yu Jeong

AU - Shang, Yun

AU - Kim, Beg Hab

AU - Kim, Sun Young

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AU - Lee, June Seung

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AU - Li, Jianming

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