Band-type microelectrodes for amperometric immunoassays

Ga Yeon Lee, Young Wook Chang, Hyuk Ko, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A band-type microelectrode was made using a parylene-N film as a passivation layer. A circular-type, mm-scale electrode with the same diameter as the band-type microelectrode was also made with an electrode area that was 5000 times larger than the band-type microelectrode. By comparing the amperometric signals of 3,5,3',5'-tetramethylbenzidine (TMB) samples at different optical density (OD) values, the band-type microelectrode was determined to be 9 times more sensitive than the circular-type electrode. The properties of the circular-type and the band-type electrodes (e.g., the shape of their cyclic voltammograms, the type of diffusion layer used, and the diffusion layer thickness per unit electrode area) were characterized according to their electrode area using the COMSOL Multiphysics software. From these simulations, the band-type electrode was estimated to have the conventional microelectrode properties, even when the electrode area was 100 times larger than a conventional circular-type electrode. These results show that both the geometry and the area of an electrode can influence the properties of the electrode. Finally, amperometric analysis based on a band-type electrode was applied to commercial ELISA kits to analyze human hepatitis B surface antigen (hHBsAg) and human immunodeficiency virus (HIV) antibodies.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalAnalytica Chimica Acta
Volume928
DOIs
Publication statusPublished - 2016 Jul 20

Fingerprint

Microelectrodes
immunoassay
Immunoassay
Electrodes
electrode
hepatitis
Density (optical)
human immunodeficiency virus
Hepatitis B Surface Antigens
antigen
Viruses
Passivation
antibody
Software
Enzyme-Linked Immunosorbent Assay
HIV

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Lee, Ga Yeon ; Chang, Young Wook ; Ko, Hyuk ; Kang, Min Jung ; Pyun, Jae Chul. / Band-type microelectrodes for amperometric immunoassays. In: Analytica Chimica Acta. 2016 ; Vol. 928. pp. 39-48.
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Band-type microelectrodes for amperometric immunoassays. / Lee, Ga Yeon; Chang, Young Wook; Ko, Hyuk; Kang, Min Jung; Pyun, Jae Chul.

In: Analytica Chimica Acta, Vol. 928, 20.07.2016, p. 39-48.

Research output: Contribution to journalArticle

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