Abstract
Emerging tissue transformation technologies provide an unprecedented opportunity to investigate system-level molecular and anatomical features in situ. Hydrogel-based methods engineer physicochemical tissue properties to render intact organs optically transparent and size and shape adjustable while preserving biomolecules at their physiological locations. When combined with advanced molecular tools, labeling, and imaging techniques, tissue transformation enables three-dimensional (3D) mapping of molecules, cells, and their interrelationships at increasing speeds and resolutions. In this review, we discuss the basic engineering principles of tissue transformation and labeling techniques as well as their broad applications, current challenges, and future potential.
| Original language | English |
|---|---|
| Pages (from-to) | 4115-4136 |
| Number of pages | 22 |
| Journal | Cell |
| Volume | 184 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2021 Aug 5 |
Bibliographical note
Funding Information:We thank the entire Chung Laboratory for support and discussions. We thank Heejin Ahn for the help in generating the mouse brain and spheroid illustration. K.C. was supported by the Burroughs Wellcome Fund Career Awards at the Scientific Interface, the Searle Scholars Program, the Packard Award in Science and Engineering, the NARSAD Young Investigator Award, the Institute for Basic Science grant no. IBS-R026-D1 , and the McKnight Foundation Technology Award. This work was supported by the JPB Foundation , the NCSOFT Cultural Foundation , and the NIH (grant U01MH117072 , DP2ES027992 ).
Funding Information:
We thank the entire Chung Laboratory for support and discussions. We thank Heejin Ahn for the help in generating the mouse brain and spheroid illustration. K.C. was supported by the Burroughs Wellcome Fund Career Awards at the Scientific Interface, the Searle Scholars Program, the Packard Award in Science and Engineering, the NARSAD Young Investigator Award, the Institute for Basic Science grant no. IBS-R026-D1, and the McKnight Foundation Technology Award. This work was supported by the JPB Foundation, the NCSOFT Cultural Foundation, and the NIH (grant U01MH117072, DP2ES027992). The authors declare no competing interests.
Publisher Copyright:
© 2021 Elsevier Inc.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)