Abstract
We have developed a microfluidic reactor array enabling systematic assessment of bacterial cells in a batch culture condition. This system enables creating a series of different growth conditions by preserving different growth factors inside nanoliter reactors and cultivating bacterial cells inside the reactors. From a single on-chip experiment, we determined the effects of carbon sources on bacterial phenotypes, i.e. the pattern of growth curves with Escherichia coli K-12. We demonstrated the systematic assessment of inhibition effect of antibiotics on the growth of Pseudomonas aeruginosa. The present system could be used for systematic phenotypic study of different types of bacterial cells.
| Original language | English |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 1710-1712 |
| Number of pages | 3 |
| Publication status | Published - 2011 |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2011 Oct 2 → 2011 Oct 6 |
Publication series
| Name | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Volume | 3 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Country | United States |
| City | Seattle, WA |
| Period | 11/10/2 → 11/10/6 |
All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
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Cite this
Dai, J., Yoon, S. H., Sim, H. Y., Oh, T. K., Kim, J. F., & Hong, J. W. (2011). Batch cultivation of bacterial cells in nanoliter reactor array. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1710-1712). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).