TY - GEN
T1 - Batch cultivation of bacterial cells in nanoliter reactor array
AU - Dai, J.
AU - Yoon, S. H.
AU - Sim, H. Y.
AU - Oh, T. K.
AU - Kim, J. F.
AU - Hong, J. W.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - We have developed a microfluidic reactor array enabling systematic assessment of bacterial cells in a batch culture condition. This system enables creating a series of different growth conditions by preserving different growth factors inside nanoliter reactors and cultivating bacterial cells inside the reactors. From a single on-chip experiment, we determined the effects of carbon sources on bacterial phenotypes, i.e. the pattern of growth curves with Escherichia coli K-12. We demonstrated the systematic assessment of inhibition effect of antibiotics on the growth of Pseudomonas aeruginosa. The present system could be used for systematic phenotypic study of different types of bacterial cells.
AB - We have developed a microfluidic reactor array enabling systematic assessment of bacterial cells in a batch culture condition. This system enables creating a series of different growth conditions by preserving different growth factors inside nanoliter reactors and cultivating bacterial cells inside the reactors. From a single on-chip experiment, we determined the effects of carbon sources on bacterial phenotypes, i.e. the pattern of growth curves with Escherichia coli K-12. We demonstrated the systematic assessment of inhibition effect of antibiotics on the growth of Pseudomonas aeruginosa. The present system could be used for systematic phenotypic study of different types of bacterial cells.
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M3 - Conference contribution
AN - SCOPUS:84883785003
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1710
EP - 1712
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -