Abstract
We have developed a microfluidic reactor array enabling systematic assessment of bacterial cells in a batch culture condition. This system enables creating a series of different growth conditions by preserving different growth factors inside nanoliter reactors and cultivating bacterial cells inside the reactors. From a single on-chip experiment, we determined the effects of carbon sources on bacterial phenotypes, i.e. the pattern of growth curves with Escherichia coli K-12. We demonstrated the systematic assessment of inhibition effect of antibiotics on the growth of Pseudomonas aeruginosa. The present system could be used for systematic phenotypic study of different types of bacterial cells.
| Original language | English |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 1710-1712 |
| Number of pages | 3 |
| Publication status | Published - 2011 Dec 1 |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2011 Oct 2 → 2011 Oct 6 |
Publication series
| Name | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Volume | 3 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Country | United States |
| City | Seattle, WA |
| Period | 11/10/2 → 11/10/6 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
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Batch cultivation of bacterial cells in nanoliter reactor array. / Dai, J.; Yoon, S. H.; Sim, H. Y.; Oh, T. K.; Kim, J. F.; Hong, J. W.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1710-1712 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Batch cultivation of bacterial cells in nanoliter reactor array
AU - Dai, J.
AU - Yoon, S. H.
AU - Sim, H. Y.
AU - Oh, T. K.
AU - Kim, J. F.
AU - Hong, J. W.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - We have developed a microfluidic reactor array enabling systematic assessment of bacterial cells in a batch culture condition. This system enables creating a series of different growth conditions by preserving different growth factors inside nanoliter reactors and cultivating bacterial cells inside the reactors. From a single on-chip experiment, we determined the effects of carbon sources on bacterial phenotypes, i.e. the pattern of growth curves with Escherichia coli K-12. We demonstrated the systematic assessment of inhibition effect of antibiotics on the growth of Pseudomonas aeruginosa. The present system could be used for systematic phenotypic study of different types of bacterial cells.
AB - We have developed a microfluidic reactor array enabling systematic assessment of bacterial cells in a batch culture condition. This system enables creating a series of different growth conditions by preserving different growth factors inside nanoliter reactors and cultivating bacterial cells inside the reactors. From a single on-chip experiment, we determined the effects of carbon sources on bacterial phenotypes, i.e. the pattern of growth curves with Escherichia coli K-12. We demonstrated the systematic assessment of inhibition effect of antibiotics on the growth of Pseudomonas aeruginosa. The present system could be used for systematic phenotypic study of different types of bacterial cells.
UR - http://www.scopus.com/inward/record.url?scp=84883785003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883785003&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883785003
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1710
EP - 1712
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ER -