Batch cultivation of bacterial cells in nanoliter reactor array

J. Dai, S. H. Yoon, H. Y. Sim, T. K. Oh, Jihyun F. Kim, J. W. Hong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a microfluidic reactor array enabling systematic assessment of bacterial cells in a batch culture condition. This system enables creating a series of different growth conditions by preserving different growth factors inside nanoliter reactors and cultivating bacterial cells inside the reactors. From a single on-chip experiment, we determined the effects of carbon sources on bacterial phenotypes, i.e. the pattern of growth curves with Escherichia coli K-12. We demonstrated the systematic assessment of inhibition effect of antibiotics on the growth of Pseudomonas aeruginosa. The present system could be used for systematic phenotypic study of different types of bacterial cells.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1710-1712
Number of pages3
Volume3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

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Antibiotics
Microfluidics
Escherichia coli
Carbon
Experiments
Intercellular Signaling Peptides and Proteins

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Dai, J., Yoon, S. H., Sim, H. Y., Oh, T. K., Kim, J. F., & Hong, J. W. (2011). Batch cultivation of bacterial cells in nanoliter reactor array. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 3, pp. 1710-1712)
Dai, J. ; Yoon, S. H. ; Sim, H. Y. ; Oh, T. K. ; Kim, Jihyun F. ; Hong, J. W. / Batch cultivation of bacterial cells in nanoliter reactor array. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. pp. 1710-1712
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Dai, J, Yoon, SH, Sim, HY, Oh, TK, Kim, JF & Hong, JW 2011, Batch cultivation of bacterial cells in nanoliter reactor array. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 1710-1712, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.

Batch cultivation of bacterial cells in nanoliter reactor array. / Dai, J.; Yoon, S. H.; Sim, H. Y.; Oh, T. K.; Kim, Jihyun F.; Hong, J. W.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 1710-1712.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Dai J, Yoon SH, Sim HY, Oh TK, Kim JF, Hong JW. Batch cultivation of bacterial cells in nanoliter reactor array. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3. 2011. p. 1710-1712