Paper-based analytical devices (e.g. lateral flow assays) are highly advantageous as portable diagnostic systems owing to their low costs and ease of use. Because of their low sensitivity and detection limits for biomolecules, these devices have several limitations in applications for real-field diagnosis. Here, we demonstrate a paper-based preconcentration enhanced lateral flow assay using a commercial β-hCG-based test. Utilizing a simple 9 V battery operation with a low power consumption of approximately 81 μW, we acquire a 25-fold preconcentration factor, demonstrating a clear sensitivity enhancement in the colorimetric lateral flow assay; consequently, clear colors are observed in a rapid kit test line, which cannot be monitored without preconcentration. This device can also facilitate a semi-quantitative platform using the saturation value and/or color intensity in both paper-based colorimetric assays and smartphone-based diagnostics.
Bibliographical noteFunding Information:
The authors are very grateful for the financial support from Korea Health Industry Development Institute (KHIDI, HI14C3319) and the National Research Foundation of Korea, Grant (NRF-2015R1D1A1A01059806). J. H. Lee was also supported by a research grant from Kwangwoon University in 2017. S. Chung was supported by the National Research Foundation of Korea (NRF-2017R1A2B3007701)
All Science Journal Classification (ASJC) codes
- Biomedical Engineering