Bax accelerates staurosporine-induced but suppresses nigericin-induced neuronal cell death

Jae H. Oh, Karen L. O'Malley, Stanislaw Krajewski, John C. Reed, Young Jun Oh

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

BAX, a member of the Bcl-2 multigene family, is known to promote apoptosis. To investigate the role of Bax in an experimentally induced cell death of the murine dopaminergic neuronal cell line (MN9D), we established MN9D cells stably over-expressing murine Bax (MN9D/Bax) or vector alone (MN9D/Neo). In MN9D/Neo cells treated with either 1 μM staurosporine or 0.1 μM nigericin, a ladder pattern of DNA fragmentation was induced. As expected, over-expression of Bax in MN9D cells accelerated staurosporine- induced cell death as measured by the MTT reduction assay (62.3% survival in MN9D/Neo vs 27.0% survival in MN9D/Bax). Surprizingly, both nigericin- induced cell death and its accompanying DNA fragmentation were largely attenuated in MN9D/Bax cells (22.0% survival in MN9D/Neo vs 86.7% survival in MN9D/Bax). Similar patterns were observed in two other MN9D/Bax cell lines. Cleavage of poly(ADP-ribose)polymerase caused by nigericin was greatly attenuated in MN9D/Bax cells suggesting that, like Bcl-2, Bax suppresses nigericin-induced cell death by inhibiting the activation of cysteine proteases. Thus, our data imply that Bax acts as a negative or positive regulator of cell death depending on the type of death stimulus applied to the cell.

Original languageEnglish
Pages (from-to)1851-1856
Number of pages6
JournalNeuroReport
Volume8
Issue number8
DOIs
Publication statusPublished - 1997 Jan 1

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Nigericin
Staurosporine
Cell Death
DNA Fragmentation
Cell Line
Cysteine Proteases
Poly(ADP-ribose) Polymerases
Multigene Family
Cell Survival
Apoptosis

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Oh, Jae H. ; O'Malley, Karen L. ; Krajewski, Stanislaw ; Reed, John C. ; Oh, Young Jun. / Bax accelerates staurosporine-induced but suppresses nigericin-induced neuronal cell death. In: NeuroReport. 1997 ; Vol. 8, No. 8. pp. 1851-1856.
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Bax accelerates staurosporine-induced but suppresses nigericin-induced neuronal cell death. / Oh, Jae H.; O'Malley, Karen L.; Krajewski, Stanislaw; Reed, John C.; Oh, Young Jun.

In: NeuroReport, Vol. 8, No. 8, 01.01.1997, p. 1851-1856.

Research output: Contribution to journalArticle

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AU - O'Malley, Karen L.

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