BBS7–SHH Signaling Activity Regulates Primary Cilia for Periodontal Homeostasis

Pi En Chang, Shujin Li, Hyun Yi Kim, Dong Joon Lee, Yoon Jeong Choi, Han Sung Jung

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Objectives: Mechanical stimuli are essential for the maintenance of periodontal ligament (PDL) homeostasis. Although there are several studies on atrophic changes in PDL due to occlusal hypofunction, the underlying mechanism is still unknown. Here, we aimed to explore the changes of gene expression in occlusal hypofunctional PDL and elucidate the related role in maintaining the PDL homeostasis. Methods: To investigate the transcriptomic difference between control and hypofunctional PDL tissue from patients, RNA sequencing was performed on 34 human teeth. The atrophic changes in PDL were evaluated by histological analysis. The effect of the Bardet-Biedl syndrome 7 (BBS7) knockdown was evaluated by the RT-qPCR, Western blot, wound healing, and tubule formation assay. Results: We detected that the expression of BBS7 was downregulated in occlusal hypofunctional PDL through RNA sequencing. Dynamic changes, including the number of periodontal ligament cells, alignment of collagen fibers, diameter of blood vessels, appearance of primary cilia, and torturous oxytalan fibers, were observed following occlusal hypofunction. Furthermore, Sonic hedgehog signaling (Shh) activity was closely associated with BBS7 expression in PDL cells. In addition, the cell migration and angiogenesis were also suppressed by BBS7 knockdown in vitro. Conclusion: We suggest that BBS7 plays an essential role in maintaining Shh signaling activity for PDL homeostasis.

Original languageEnglish
Article number796274
JournalFrontiers in Cell and Developmental Biology
Publication statusPublished - 2021 Dec 7

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean government (MSIT) (No. NRF-2017R1C1B5017498) and the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. NRF-2019R1A2C3005294).

Publisher Copyright:
Copyright © 2021 Chang, Li, Kim, Lee, Choi and Jung.

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology


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