Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity

Jong Wook Park, Yun Jung Choi, Seong Il Suh, Won Ki Baek, Min Ho Suh, Ing Nyol Jin, Do Sik Min, Ju Hyung Woo, Jong Soo Chang, Antonino Passaniti, Young Han Lee, Taeg Kyu Kwon

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Resveratrol has been shown to induce anti-proliferation and apoptosis of human cancer cell lines. In the present study, we determined the effect of high intracellular levels of the anti-apoptosis protein Bcl-2 on caspase-3 activation, PLC-γ1 degradation and cytochrome c release during resveratrol-induced apoptosis. For this, we used U937/vector and U937/Bcl-2 cells, which were generated by transfection of the cDNA of the Bcl-2 gene. As compared with U937/vector, U937/Bcl-2 cells exhibited a 4-fold greater expression of Bcl-2. Treatment with 60 or 100 μM resveratrol for 24 h produced morphological features of apoptosis and DNA fragmentation in U937/vector cells, respectively. This was associated with caspase-3 activation and PLC-γ1 degradation. In contrast, resveratrol-induced caspase-3 activation and PLC-γ1 degradation and apoptosis were significantly inhibited in U937/Bcl-2 cells. Bcl-2 overexpressing cells exhibited less cytochrome c release and sustained expression levels of the IAP proteins during resveratrol-induced apoptosis. In addition, these findings indicate that Bcl-2 inhibits resveratrol-induced apoptosis by a mechanism that interferes with cytochrome c release and activity of caspase-3 that is involved in the execution of apoptosis.

Original languageEnglish
Pages (from-to)1633-1639
Number of pages7
JournalCarcinogenesis
Volume22
Issue number10
DOIs
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Cancer Research

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