Behçet's Disease Sera Containing Antiendothelial Cell Antibodies Promote Adhesion of T Lymphocytes to Cultured Human Dermal Microvascular Endothelial Cells

Kwang Hoon Lee, Hae Shin Chung, Dongsik Bang, Sungnack Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Antiendothelial cell antibodies (AECA) have been detected in the sera of patients of autoimmune diseases showing vasculitis. Using IgM-ELISA, we found AECA in 42 (56%) of 75 sera samples from patients with Behçet's disease in a previous study. All of the 15 AECA-positive sera of Behçet's disease patients had an increased expression of the intercellular cell adhesion molecule-1 (ICAM-1), 93.3% of the sera induced the vascular cell adhesion molecule-1 (VCAM-1), and 100% of the serum induced the E-selectin molecule on human dermal microvascular endothelial cells (HDMEC). After stimulation of HDMEC with AECA-positive sera of Behçet's disease patients, the expression of ICAM-1 and VCAM-1 on HDMEC increased significantly at 4 hours, reaching a peak at 16 hours. Expression of E-selectin was induced at 1 hour after stimulation with a peak at 4 hours and it decreased thereafter. Adherence of T lymphocytes to HDMEC increased significantly after stimulation with AECA-positive sera from Behçet's disease patients. Also, the adherence of T lymphocytes to HDMEC increased at 4 hours and returned to its normal level at 48 hours. These results show that AECA-positive sera of Behçet's disease patients are capable of activating HDMEC to promote the adherence of T lymphocytes to increase the expression of ICAM-1, VCAM-1, and E-selectin on the cell surfaces. The whole process may play an important role in the pathogenesis of vasculitis in Behçet's disease.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalYonsei medical journal
Volume40
Issue number2
Publication statusPublished - 1999 Apr 1

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Endothelial Cells
T-Lymphocytes
Skin
Serum
E-Selectin
Vascular Cell Adhesion Molecule-1
Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Vasculitis
anti-endothelial cell antibody
Autoimmune Diseases
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{6c4b138e5a614b09b0d3c3c52716a057,
title = "Beh{\cc}et's Disease Sera Containing Antiendothelial Cell Antibodies Promote Adhesion of T Lymphocytes to Cultured Human Dermal Microvascular Endothelial Cells",
abstract = "Antiendothelial cell antibodies (AECA) have been detected in the sera of patients of autoimmune diseases showing vasculitis. Using IgM-ELISA, we found AECA in 42 (56{\%}) of 75 sera samples from patients with Beh{\cc}et's disease in a previous study. All of the 15 AECA-positive sera of Beh{\cc}et's disease patients had an increased expression of the intercellular cell adhesion molecule-1 (ICAM-1), 93.3{\%} of the sera induced the vascular cell adhesion molecule-1 (VCAM-1), and 100{\%} of the serum induced the E-selectin molecule on human dermal microvascular endothelial cells (HDMEC). After stimulation of HDMEC with AECA-positive sera of Beh{\cc}et's disease patients, the expression of ICAM-1 and VCAM-1 on HDMEC increased significantly at 4 hours, reaching a peak at 16 hours. Expression of E-selectin was induced at 1 hour after stimulation with a peak at 4 hours and it decreased thereafter. Adherence of T lymphocytes to HDMEC increased significantly after stimulation with AECA-positive sera from Beh{\cc}et's disease patients. Also, the adherence of T lymphocytes to HDMEC increased at 4 hours and returned to its normal level at 48 hours. These results show that AECA-positive sera of Beh{\cc}et's disease patients are capable of activating HDMEC to promote the adherence of T lymphocytes to increase the expression of ICAM-1, VCAM-1, and E-selectin on the cell surfaces. The whole process may play an important role in the pathogenesis of vasculitis in Beh{\cc}et's disease.",
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Behçet's Disease Sera Containing Antiendothelial Cell Antibodies Promote Adhesion of T Lymphocytes to Cultured Human Dermal Microvascular Endothelial Cells. / Lee, Kwang Hoon; Chung, Hae Shin; Bang, Dongsik; Lee, Sungnack.

In: Yonsei medical journal, Vol. 40, No. 2, 01.04.1999, p. 152-158.

Research output: Contribution to journalArticle

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T1 - Behçet's Disease Sera Containing Antiendothelial Cell Antibodies Promote Adhesion of T Lymphocytes to Cultured Human Dermal Microvascular Endothelial Cells

AU - Lee, Kwang Hoon

AU - Chung, Hae Shin

AU - Bang, Dongsik

AU - Lee, Sungnack

PY - 1999/4/1

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N2 - Antiendothelial cell antibodies (AECA) have been detected in the sera of patients of autoimmune diseases showing vasculitis. Using IgM-ELISA, we found AECA in 42 (56%) of 75 sera samples from patients with Behçet's disease in a previous study. All of the 15 AECA-positive sera of Behçet's disease patients had an increased expression of the intercellular cell adhesion molecule-1 (ICAM-1), 93.3% of the sera induced the vascular cell adhesion molecule-1 (VCAM-1), and 100% of the serum induced the E-selectin molecule on human dermal microvascular endothelial cells (HDMEC). After stimulation of HDMEC with AECA-positive sera of Behçet's disease patients, the expression of ICAM-1 and VCAM-1 on HDMEC increased significantly at 4 hours, reaching a peak at 16 hours. Expression of E-selectin was induced at 1 hour after stimulation with a peak at 4 hours and it decreased thereafter. Adherence of T lymphocytes to HDMEC increased significantly after stimulation with AECA-positive sera from Behçet's disease patients. Also, the adherence of T lymphocytes to HDMEC increased at 4 hours and returned to its normal level at 48 hours. These results show that AECA-positive sera of Behçet's disease patients are capable of activating HDMEC to promote the adherence of T lymphocytes to increase the expression of ICAM-1, VCAM-1, and E-selectin on the cell surfaces. The whole process may play an important role in the pathogenesis of vasculitis in Behçet's disease.

AB - Antiendothelial cell antibodies (AECA) have been detected in the sera of patients of autoimmune diseases showing vasculitis. Using IgM-ELISA, we found AECA in 42 (56%) of 75 sera samples from patients with Behçet's disease in a previous study. All of the 15 AECA-positive sera of Behçet's disease patients had an increased expression of the intercellular cell adhesion molecule-1 (ICAM-1), 93.3% of the sera induced the vascular cell adhesion molecule-1 (VCAM-1), and 100% of the serum induced the E-selectin molecule on human dermal microvascular endothelial cells (HDMEC). After stimulation of HDMEC with AECA-positive sera of Behçet's disease patients, the expression of ICAM-1 and VCAM-1 on HDMEC increased significantly at 4 hours, reaching a peak at 16 hours. Expression of E-selectin was induced at 1 hour after stimulation with a peak at 4 hours and it decreased thereafter. Adherence of T lymphocytes to HDMEC increased significantly after stimulation with AECA-positive sera from Behçet's disease patients. Also, the adherence of T lymphocytes to HDMEC increased at 4 hours and returned to its normal level at 48 hours. These results show that AECA-positive sera of Behçet's disease patients are capable of activating HDMEC to promote the adherence of T lymphocytes to increase the expression of ICAM-1, VCAM-1, and E-selectin on the cell surfaces. The whole process may play an important role in the pathogenesis of vasculitis in Behçet's disease.

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