Bench-scale ex situ diesel removal process using a biobarrier and surfactant flushing

Young Chul Lee, Sung Geun Woo, Eun Sil Choi, Yeonghee Ahn, Joonhong Park, Myungjin Lee, Ji Won Yang

Research output: Contribution to journalArticle

10 Citations (Scopus)


Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2×10 9 to 8×10 9 (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from ∼2×10 6 to ∼5×10 6 (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000±45mg/kg to below 5mg/kg. While 99.9%, i.e. up to below 5mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel.

Original languageEnglish
Pages (from-to)882-887
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Issue number3
Publication statusPublished - 2012 May 25

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

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