Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2×10 9 to 8×10 9 (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from ∼2×10 6 to ∼5×10 6 (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000±45mg/kg to below 5mg/kg. While 99.9%, i.e. up to below 5mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel.
Bibliographical noteFunding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology ( ABC-2010-0029728 ) and supported by the Korean Ministry of Environment as “GAIA project.” We also acknowledge the Korea Research Institute of Bioscience and Biotechnology (KRIBB) to get the bioSEM images.
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)