Abstract
Berberine, an herbal alkaloid, has been reported to have a lipid lowering effect by stabilizing hepatic LDLR mRNA in an ERK-dependent manner rather than promoting transcriptional activity. However, considering the complexity of interconnected signal pathways in biological processes, it is highly possible that there exist signal pathway(s) other than ERK pathway which contribute to the berberine-induced up-regulation of LDLR. In the present study, we examined possible involvement of other signal pathways in berberine-induced hepatic LDLR up-regulation. As evidenced by RT-PCR, berberine-induced LDLR mRNA expression was inhibited by JNK inhibitor SP600125 pretreatment. Furthermore, we demonstrate that putative c-jun binding site of LDLR promoter is important in berberine-induced LDLR transcription using luciferase assay. The result of EMSA also shows that berberine induces c-jun binding to LDLR promoter and this is decreased by SP600125 pretreatment. The present study demonstrates that berberine increases transcriptional activity of LDLR promoter and this involves JNK pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 853-857 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 362 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2007 Nov 3 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Berberine-induced LDLR up-regulation involves JNK pathway. / Lee, Seahyoung; Lim, Hyun Joung; Park, Jin Hee; Lee, Kuy Sook; Jang, Yangsoo; Park, Hyun Young.
In: Biochemical and Biophysical Research Communications, Vol. 362, No. 4, 03.11.2007, p. 853-857.Research output: Contribution to journal › Article
TY - JOUR
T1 - Berberine-induced LDLR up-regulation involves JNK pathway
AU - Lee, Seahyoung
AU - Lim, Hyun Joung
AU - Park, Jin Hee
AU - Lee, Kuy Sook
AU - Jang, Yangsoo
AU - Park, Hyun Young
PY - 2007/11/3
Y1 - 2007/11/3
N2 - Berberine, an herbal alkaloid, has been reported to have a lipid lowering effect by stabilizing hepatic LDLR mRNA in an ERK-dependent manner rather than promoting transcriptional activity. However, considering the complexity of interconnected signal pathways in biological processes, it is highly possible that there exist signal pathway(s) other than ERK pathway which contribute to the berberine-induced up-regulation of LDLR. In the present study, we examined possible involvement of other signal pathways in berberine-induced hepatic LDLR up-regulation. As evidenced by RT-PCR, berberine-induced LDLR mRNA expression was inhibited by JNK inhibitor SP600125 pretreatment. Furthermore, we demonstrate that putative c-jun binding site of LDLR promoter is important in berberine-induced LDLR transcription using luciferase assay. The result of EMSA also shows that berberine induces c-jun binding to LDLR promoter and this is decreased by SP600125 pretreatment. The present study demonstrates that berberine increases transcriptional activity of LDLR promoter and this involves JNK pathway.
AB - Berberine, an herbal alkaloid, has been reported to have a lipid lowering effect by stabilizing hepatic LDLR mRNA in an ERK-dependent manner rather than promoting transcriptional activity. However, considering the complexity of interconnected signal pathways in biological processes, it is highly possible that there exist signal pathway(s) other than ERK pathway which contribute to the berberine-induced up-regulation of LDLR. In the present study, we examined possible involvement of other signal pathways in berberine-induced hepatic LDLR up-regulation. As evidenced by RT-PCR, berberine-induced LDLR mRNA expression was inhibited by JNK inhibitor SP600125 pretreatment. Furthermore, we demonstrate that putative c-jun binding site of LDLR promoter is important in berberine-induced LDLR transcription using luciferase assay. The result of EMSA also shows that berberine induces c-jun binding to LDLR promoter and this is decreased by SP600125 pretreatment. The present study demonstrates that berberine increases transcriptional activity of LDLR promoter and this involves JNK pathway.
UR - http://www.scopus.com/inward/record.url?scp=34548591723&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548591723&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.08.060
DO - 10.1016/j.bbrc.2007.08.060
M3 - Article
C2 - 17767919
AN - SCOPUS:34548591723
VL - 362
SP - 853
EP - 857
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -