Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells

Yun Min Kook, Young Mi Kang, Seong Hwan Moon, Won Gun Koh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmental hydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogel domain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the bare alginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiber-incorporated hydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluorescence staining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups produced more collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than the single-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It is expected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for the study of various cell-cell interactions.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalJournal of Industrial and Engineering Chemistry
Volume38
DOIs
Publication statusPublished - 2016 Jun 25

Fingerprint

Hydrogel
Scaffolds (biology)
Stem cells
Cell culture
Hydrogels
Alginate
Bone
A73025
Nanofibers
Aggrecans
Collagen Type II
Facilitative Glucose Transport Proteins
Polymerase chain reaction
Electrospinning
Gelation
Glycosaminoglycans
Collagen
Scaffolds
Glucose
Assays

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

Cite this

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abstract = "The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmental hydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogel domain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the bare alginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiber-incorporated hydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluorescence staining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups produced more collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than the single-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It is expected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for the study of various cell-cell interactions.",
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Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells. / Kook, Yun Min; Kang, Young Mi; Moon, Seong Hwan; Koh, Won Gun.

In: Journal of Industrial and Engineering Chemistry, Vol. 38, 25.06.2016, p. 113-122.

Research output: Contribution to journalArticle

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AB - The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmental hydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogel domain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the bare alginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiber-incorporated hydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluorescence staining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups produced more collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than the single-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It is expected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for the study of various cell-cell interactions.

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