Binary Phosphorene Redox Behavior in Oxidoreductase Enzymatic Systems

Carmen C. Mayorga-Martinez, Zdeněk Sofer, Martin Pumera

Research output: Contribution to journalArticle

Abstract

Phosphorene is a two-dimensional material that has many advantageous electronic, electrochemical, and optical properties. However, phosphorene possesses a relatively poor stability in ambient atmosphere. This disadvantage limits its application in several systems and particularly in electrochemical biosensors. Here we evaluate phosphorene as an electrochemical biosensing platform in two different mediator-based oxidoreductase enzymatic systems (glucose oxidase (GOx) and peroxidase from horseradish (HRP)), in which their detection is based on the reduction or oxidation of a mediator. In both cases, the used mediator is the same, ferrocene methanol (FcMeOH). Enhanced electrochemical activity is observed only in the reductive system (HRP-based biosensor) when compared to the oxidative counterpart (GOx-based biosensor). This phenomenon is attributed to the fact that in a reductive environment the phosphorene structure remains intact, while in an oxidative potential, the phosphorene is readily oxidized. In this way, the electroactivity of phosphorene as a sensing platform is strongly dependent on the type of mediator-based enzymatic system. These findings of binary nature of phosphorene are of high importance for construction of phosphorene-sensing platforms and in the development of enzyme logic systems.

Original languageEnglish
Pages (from-to)13217-13224
Number of pages8
JournalACS Nano
Volume13
Issue number11
DOIs
Publication statusPublished - 2019 Nov 26

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bioinstrumentation
Biosensors
Glucose Oxidase
Oxidoreductases
Glucose oxidase
platforms
oxidase
glucose
Electrochemical properties
Electronic properties
logic
Methanol
enzymes
Enzymes
methyl alcohol
Optical properties
optical properties
atmospheres
Oxidation
oxidation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Mayorga-Martinez, Carmen C. ; Sofer, Zdeněk ; Pumera, Martin. / Binary Phosphorene Redox Behavior in Oxidoreductase Enzymatic Systems. In: ACS Nano. 2019 ; Vol. 13, No. 11. pp. 13217-13224.
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Binary Phosphorene Redox Behavior in Oxidoreductase Enzymatic Systems. / Mayorga-Martinez, Carmen C.; Sofer, Zdeněk; Pumera, Martin.

In: ACS Nano, Vol. 13, No. 11, 26.11.2019, p. 13217-13224.

Research output: Contribution to journalArticle

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