Bio-inspired oligovitronectin-grafted surface for enhanced self-renewal and long-term maintenance of human pluripotent stem cells under feeder-free conditions

Hyun Ji Park, Kisuk Yang, Mun Jung Kim, Jiho Jang, Mihyun Lee, Dong Wook Kim, Haeshin Lee, Seung Woo Cho

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Current protocols for human pluripotent stem cell (hPSC) expansion require feeder cells or matrices from animal sources that have been the major obstacle to obtain clinical grade hPSCs due to safety issues, difficulty in quality control, and high expense. Thus, feeder-free, chemically defined synthetic platforms have been developed, but are mostly confined to typical polystyrene culture plates. Here, we report a chemically defined, material-independent, bio-inspired surface coating allowing for feeder-free expansion and maintenance of self-renewal and pluripotency of hPSCs on various polymer substrates and devices. Polydopamine (pDA)-mediated immobilization of vitronectin (VN) peptides results in surface functionalization of VN-dimer/pDA conjugates. The engineered surfaces facilitate adhesion, proliferation, and colony formation of hPSCs via enhanced focal adhesion, cell-cell interaction, and biophysical signals, providing a chemically defined, xeno-free culture system for clonal expansion and long-term maintenance of hPSCs. This surface engineering enables the application of clinically-relevant hPSCs to a variety of biomedical systems such as tissue-engineering scaffolds and medical devices.

Original languageEnglish
Pages (from-to)127-139
Number of pages13
JournalBiomaterials
Volume50
Issue number1
DOIs
Publication statusPublished - 2015

Bibliographical note

Funding Information:
This work was supported by grants ( NRF-2010-0020409 and NRF-2013R1A1A2A10061422 ) from the National Research Foundation of Korea (NRF) and a grant ( 2009-0083522 ) from the Translational Research Center for Protein Function Control (TRCP) funded by the Ministry of Science, ICT and Future Planning (MSIP), Republic of Korea. This work was also supported by a grant ( HI13C1479 ) from the Korea Health Technology R&D Project funded by the Ministry of Health and Welfare, Republic of Korea. We appreciate Jin Kim for her assistance in preparing schematic illustrations in Fig. 1 .

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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