Biochemical characteristics of chitinase enzyme from Bacillus sp. of Kamojang Crater, Indonesia

Hasnah Natsir, Debbie Chandra, Yaya Rukayadi, Maggy Thenawidjaja Suhartono, Jae Kwan Hwang, Yu Ryang Pyun

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Chitinase and chitindeacetylase are enzymes capable of degrading chitin into chitooligomers and chitosan. The chitinases characterized and purified in this study were extracted from the acidophilic Bacillus sp. isolated from Kamojang Crater West Java Indonesia. When grown in liquid media containing colloidal chitin, the optimum chitinase activity of the acidophilic isolate was reached after 4-5 days of incubation. The optimum temperature and pH of the chitinase and chitin deacetylase were found at 37°C and pH 5. When incubated at pH 5, the activity of chitin deacetylase was increased; after 3 h, the activity was 1.5 times of the control. The enzyme was stable at pH 4, after 2 h incubation, the activity was still 80% of the control. The chitinase and chitin deacetylase activities were not influenced by Mg++ nor Ca++, Ni++ and Cu++ inhibited the chitinase activity, while chitin deacetylase activity was not affected by Cu++ addition. When 1 mM of EDTA was added, the enzyme activity was reduced 40 to 50%.

Original languageEnglish
Pages (from-to)279-282
Number of pages4
JournalJournal of Biochemistry, Molecular Biology and Biophysics
Volume6
Issue number4
Publication statusPublished - 2002 Aug 1

Fingerprint

chitin deacetylase
Chitinases
Indonesia
Bacilli
Bacillus
Enzymes
Chitin
Chitosan
Enzyme activity
Edetic Acid
Temperature
Liquids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Natsir, Hasnah ; Chandra, Debbie ; Rukayadi, Yaya ; Suhartono, Maggy Thenawidjaja ; Hwang, Jae Kwan ; Pyun, Yu Ryang. / Biochemical characteristics of chitinase enzyme from Bacillus sp. of Kamojang Crater, Indonesia. In: Journal of Biochemistry, Molecular Biology and Biophysics. 2002 ; Vol. 6, No. 4. pp. 279-282.
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Biochemical characteristics of chitinase enzyme from Bacillus sp. of Kamojang Crater, Indonesia. / Natsir, Hasnah; Chandra, Debbie; Rukayadi, Yaya; Suhartono, Maggy Thenawidjaja; Hwang, Jae Kwan; Pyun, Yu Ryang.

In: Journal of Biochemistry, Molecular Biology and Biophysics, Vol. 6, No. 4, 01.08.2002, p. 279-282.

Research output: Contribution to journalArticle

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AU - Hwang, Jae Kwan

AU - Pyun, Yu Ryang

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