Biodegradable polyester, poly[α-(4-Aminobutyl)-L-glycolic acid], as a non-toxic gene carrier

Yong-beom Lim, Sang Oh Han, Han Uk Kong, Yah Lee, Jong Sang Park, Byeongmoon Jeong, Sung Wan Kim

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Purpose. The aim of this study was to develop a non-toxic polymeric gene carrier. For this purpose, biodegradable cationic polymer, poly[α-(4-aminobutyl)-L-glycolic acid] (PAGA) was synthesized. PAGA was designed to have ester linkage because polyesters usually show biodegradability. Methods. Degradation of PAGA in an aqueous solution was followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). PAGA/DNA complexes were characterized by gel electrophoresis, atomic force microscopy (AFM), dynamic light scattering (DLS). The transfection was measured by using the β-galactosidase reporter gene. Results. PAGA was degraded in aqueous solution very quickly and the final degradation product was a monomer (L-oxylysine). Formation of self-assembling biodegradable complexes between PAGA and DNA at a charge ratio 1:1 (+/-) was confirmed by gel band shift assay and AFM. In these studies, controlled release of DNA from the complexes could be seen. The complexes showed about 2-fold higher transfection efficiency than DNA complexes of poly-L-lysine (PLL), a structural analogue of PAGA, which is the most commonly used poly-cation for gene delivery. The polymer did not show cytotoxicity, possibly because of its degradability and the biocompatibility of the monomer. Conclusions. The use of the biodegradable poly-cation, PAGA, as a DNA condensing agent will be useful in safe gene delivery.

Original languageEnglish
Pages (from-to)811-816
Number of pages6
JournalPharmaceutical Research
Volume17
Issue number7
DOIs
Publication statusPublished - 2000 Jan 1

Fingerprint

glycolic acid
Polyesters
Genes
DNA
Atomic Force Microscopy
Transfection
Cations
Atomic force microscopy
Polymers
Monomers
Gels
Galactosidases
Degradation
Biodegradability
Dynamic light scattering
Cytotoxicity
Electrophoresis
Biocompatibility
Reporter Genes
Lysine

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

Cite this

Lim, Yong-beom ; Han, Sang Oh ; Kong, Han Uk ; Lee, Yah ; Park, Jong Sang ; Jeong, Byeongmoon ; Kim, Sung Wan. / Biodegradable polyester, poly[α-(4-Aminobutyl)-L-glycolic acid], as a non-toxic gene carrier. In: Pharmaceutical Research. 2000 ; Vol. 17, No. 7. pp. 811-816.
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abstract = "Purpose. The aim of this study was to develop a non-toxic polymeric gene carrier. For this purpose, biodegradable cationic polymer, poly[α-(4-aminobutyl)-L-glycolic acid] (PAGA) was synthesized. PAGA was designed to have ester linkage because polyesters usually show biodegradability. Methods. Degradation of PAGA in an aqueous solution was followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). PAGA/DNA complexes were characterized by gel electrophoresis, atomic force microscopy (AFM), dynamic light scattering (DLS). The transfection was measured by using the β-galactosidase reporter gene. Results. PAGA was degraded in aqueous solution very quickly and the final degradation product was a monomer (L-oxylysine). Formation of self-assembling biodegradable complexes between PAGA and DNA at a charge ratio 1:1 (+/-) was confirmed by gel band shift assay and AFM. In these studies, controlled release of DNA from the complexes could be seen. The complexes showed about 2-fold higher transfection efficiency than DNA complexes of poly-L-lysine (PLL), a structural analogue of PAGA, which is the most commonly used poly-cation for gene delivery. The polymer did not show cytotoxicity, possibly because of its degradability and the biocompatibility of the monomer. Conclusions. The use of the biodegradable poly-cation, PAGA, as a DNA condensing agent will be useful in safe gene delivery.",
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Biodegradable polyester, poly[α-(4-Aminobutyl)-L-glycolic acid], as a non-toxic gene carrier. / Lim, Yong-beom; Han, Sang Oh; Kong, Han Uk; Lee, Yah; Park, Jong Sang; Jeong, Byeongmoon; Kim, Sung Wan.

In: Pharmaceutical Research, Vol. 17, No. 7, 01.01.2000, p. 811-816.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biodegradable polyester, poly[α-(4-Aminobutyl)-L-glycolic acid], as a non-toxic gene carrier

AU - Lim, Yong-beom

AU - Han, Sang Oh

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AU - Lee, Yah

AU - Park, Jong Sang

AU - Jeong, Byeongmoon

AU - Kim, Sung Wan

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N2 - Purpose. The aim of this study was to develop a non-toxic polymeric gene carrier. For this purpose, biodegradable cationic polymer, poly[α-(4-aminobutyl)-L-glycolic acid] (PAGA) was synthesized. PAGA was designed to have ester linkage because polyesters usually show biodegradability. Methods. Degradation of PAGA in an aqueous solution was followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). PAGA/DNA complexes were characterized by gel electrophoresis, atomic force microscopy (AFM), dynamic light scattering (DLS). The transfection was measured by using the β-galactosidase reporter gene. Results. PAGA was degraded in aqueous solution very quickly and the final degradation product was a monomer (L-oxylysine). Formation of self-assembling biodegradable complexes between PAGA and DNA at a charge ratio 1:1 (+/-) was confirmed by gel band shift assay and AFM. In these studies, controlled release of DNA from the complexes could be seen. The complexes showed about 2-fold higher transfection efficiency than DNA complexes of poly-L-lysine (PLL), a structural analogue of PAGA, which is the most commonly used poly-cation for gene delivery. The polymer did not show cytotoxicity, possibly because of its degradability and the biocompatibility of the monomer. Conclusions. The use of the biodegradable poly-cation, PAGA, as a DNA condensing agent will be useful in safe gene delivery.

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