Biodegradation of the organochlorine insecticide, endosulfan, and the toxic metabolite, endosulfan sulfate, by Klebsiella oxytoca KE-8

Gi Seok Kwon, Ho Yong Sohn, Kee Sun Shin, Eungbin KIm, Bu Il Seo

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Biodegradation of endosulfan, a chlorinated cyclodiene insecticide, is generally accompanied by production of the more toxic and more persistent metabolite, endosulfan sulfate. Since our reported endosulfan degrader, Klebsiella pneumoniae KE-1, failed to degrade endosulfan sulfate, we tried to isolate an endosulfan sulfate degrader from endosulfan-polluted soils. Through repetitive enrichment and successive subculture using mineral salt medium containing endosulfan or endosulfan sulfate as the sole source of carbon and energy, we isolated a bacterium capable of degrading endosulfan sulfate as well as endosulfan. The bacterium KE-8 was identified as Klebsiella oxytoca from the results of 16S rDNA sequence analysis. In biodegradation assays with KE-8 using mineral salt medium containing endosulfan (150 mg l-1) or endosulfan sulfate (173 mg l-1), the biomass was rapidly increased to an optical density at 550 nm of 1.9 in 4 days and the degradation constants for α- and β-endosulfan, and endosulfan sulfate were 0.3084, 0.2983 and 0.2465 day-1, respectively. Analysis of the metabolites further suggested that K. oxytoca KE-8 has high potential as a biocatalyst for bioremediation of endosulfan and/or endosulfan sulfate.

Original languageEnglish
Pages (from-to)845-850
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume67
Issue number6
DOIs
Publication statusPublished - 2005 Jun 1

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Klebsiella oxytoca
Endosulfan
Poisons
Insecticides
Minerals
Salts
Bacteria
Environmental Biodegradation
endosulfan sulfate
Klebsiella pneumoniae
Ribosomal DNA
Biomass
Sequence Analysis
Soil
Carbon

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Biodegradation of the organochlorine insecticide, endosulfan, and the toxic metabolite, endosulfan sulfate, by Klebsiella oxytoca KE-8",
abstract = "Biodegradation of endosulfan, a chlorinated cyclodiene insecticide, is generally accompanied by production of the more toxic and more persistent metabolite, endosulfan sulfate. Since our reported endosulfan degrader, Klebsiella pneumoniae KE-1, failed to degrade endosulfan sulfate, we tried to isolate an endosulfan sulfate degrader from endosulfan-polluted soils. Through repetitive enrichment and successive subculture using mineral salt medium containing endosulfan or endosulfan sulfate as the sole source of carbon and energy, we isolated a bacterium capable of degrading endosulfan sulfate as well as endosulfan. The bacterium KE-8 was identified as Klebsiella oxytoca from the results of 16S rDNA sequence analysis. In biodegradation assays with KE-8 using mineral salt medium containing endosulfan (150 mg l-1) or endosulfan sulfate (173 mg l-1), the biomass was rapidly increased to an optical density at 550 nm of 1.9 in 4 days and the degradation constants for α- and β-endosulfan, and endosulfan sulfate were 0.3084, 0.2983 and 0.2465 day-1, respectively. Analysis of the metabolites further suggested that K. oxytoca KE-8 has high potential as a biocatalyst for bioremediation of endosulfan and/or endosulfan sulfate.",
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Biodegradation of the organochlorine insecticide, endosulfan, and the toxic metabolite, endosulfan sulfate, by Klebsiella oxytoca KE-8. / Kwon, Gi Seok; Sohn, Ho Yong; Shin, Kee Sun; KIm, Eungbin; Seo, Bu Il.

In: Applied Microbiology and Biotechnology, Vol. 67, No. 6, 01.06.2005, p. 845-850.

Research output: Contribution to journalArticle

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AU - Seo, Bu Il

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