Biogeochemical dissolution of nontronite by Shewanella oneidensis MR-1

Evidence of biotic illite formation

Tae Hee Koo, Gunjeong Lee, Jin-Wook Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Microbial Fe-reduction in smectite structure plays a significant role in illitization accompanying with the structural/chemical modification of smectite, closely linked to the physico-chemical properties of clays, Fe-liberation, water chemistry, elemental cycles, and fault behavior. Biotic dissolution of smectite is a major process that promotes illitization, however direct evidence of illite formation is not clearly understood. In the present study, a combination of spectroscopic, microscopic, and chemical analyses revealed evidence of illitization in bio-reduced smectite, with elemental composition measured on a nanoscale as incubation time increased. Fe-reducing bacteria (FeRB), Shewanella oneidensis MR-1 was inoculated in M1 medium with nontronite (NAu-1) less than 0.2 μm as an electron acceptor and Na-lactate as a sole electron donor at 30 °C in the anaerobic chamber for up to 120 days. The alkalinity was maintained at pH 8.0 in the whole experiment to enhance illite formation. The extent of Fe(III) reduction measured by 1,10-phenanthroline assay reached up to 10.6% in the experiment while less than ~ 1% of reduction was measured in no-bacteria control. In biotic and abiotic control, increases of elemental concentrations (Si, Al, and Fe) in the supernatant indicated the dissolution of nontronite. The progress of bio-reduced nontronite reaction can be explained as follows: altered nontronite (AN) with a scouring surface texture → K-nontronite (KN) with frayed edges → euhedral lath shaped illite. A progressive morphology change in bio-reduced nontronite corresponded to an increase in Al/Si and K / (K + 2Ca) that ranged between 0.13 and 0.28 and 0.16 to 1.0, suggesting the biotic reductive dissolution of nontronite and neoformation of illite. The precipitation of biotic amorphous silica supported the reductive dissolution of nontronite. In contrast, there was no clear evidence of mineral precipitation in no-bacteria control. Following treatment with Li and ethylene glycol for the long-term incubation (70 and 120 days), the X-ray diffraction (XRD) profiles confirmed illitization by displaying a 10-Å peak shoulder at around 8.9° 2θ in the bio-reduced nontronite. Indeed, a direct microscopic observation of distinct illite packets of 16 nm in thickness with d001 = 1.0 nm in the wavy nontronite matrix with various spacings (d001 = 1.2–1.3 nm) strongly suggested biotic illite formation.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalApplied Clay Science
Volume134
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

nontronite
illite
Dissolution
dissolution
illitization
Bacteria
smectite
Ethylene Glycol
Electrons
Chemical modification
bacterium
Alkalinity
Silicon Dioxide
Chemical properties
incubation
Minerals
Lactic Acid
Assays
Textures
electron

All Science Journal Classification (ASJC) codes

  • Geology
  • Geochemistry and Petrology

Cite this

@article{a78c040d56de4372a8ad72a0678864ff,
title = "Biogeochemical dissolution of nontronite by Shewanella oneidensis MR-1: Evidence of biotic illite formation",
abstract = "Microbial Fe-reduction in smectite structure plays a significant role in illitization accompanying with the structural/chemical modification of smectite, closely linked to the physico-chemical properties of clays, Fe-liberation, water chemistry, elemental cycles, and fault behavior. Biotic dissolution of smectite is a major process that promotes illitization, however direct evidence of illite formation is not clearly understood. In the present study, a combination of spectroscopic, microscopic, and chemical analyses revealed evidence of illitization in bio-reduced smectite, with elemental composition measured on a nanoscale as incubation time increased. Fe-reducing bacteria (FeRB), Shewanella oneidensis MR-1 was inoculated in M1 medium with nontronite (NAu-1) less than 0.2 μm as an electron acceptor and Na-lactate as a sole electron donor at 30 °C in the anaerobic chamber for up to 120 days. The alkalinity was maintained at pH 8.0 in the whole experiment to enhance illite formation. The extent of Fe(III) reduction measured by 1,10-phenanthroline assay reached up to 10.6{\%} in the experiment while less than ~ 1{\%} of reduction was measured in no-bacteria control. In biotic and abiotic control, increases of elemental concentrations (Si, Al, and Fe) in the supernatant indicated the dissolution of nontronite. The progress of bio-reduced nontronite reaction can be explained as follows: altered nontronite (AN) with a scouring surface texture → K-nontronite (KN) with frayed edges → euhedral lath shaped illite. A progressive morphology change in bio-reduced nontronite corresponded to an increase in Al/Si and K / (K + 2Ca) that ranged between 0.13 and 0.28 and 0.16 to 1.0, suggesting the biotic reductive dissolution of nontronite and neoformation of illite. The precipitation of biotic amorphous silica supported the reductive dissolution of nontronite. In contrast, there was no clear evidence of mineral precipitation in no-bacteria control. Following treatment with Li and ethylene glycol for the long-term incubation (70 and 120 days), the X-ray diffraction (XRD) profiles confirmed illitization by displaying a 10-{\AA} peak shoulder at around 8.9° 2θ in the bio-reduced nontronite. Indeed, a direct microscopic observation of distinct illite packets of 16 nm in thickness with d001 = 1.0 nm in the wavy nontronite matrix with various spacings (d001 = 1.2–1.3 nm) strongly suggested biotic illite formation.",
author = "Koo, {Tae Hee} and Gunjeong Lee and Jin-Wook Kim",
year = "2016",
month = "12",
day = "1",
doi = "10.1016/j.clay.2016.03.030",
language = "English",
volume = "134",
pages = "13--18",
journal = "Applied Clay Science",
issn = "0169-1317",
publisher = "Elsevier BV",

}

Biogeochemical dissolution of nontronite by Shewanella oneidensis MR-1 : Evidence of biotic illite formation. / Koo, Tae Hee; Lee, Gunjeong; Kim, Jin-Wook.

In: Applied Clay Science, Vol. 134, 01.12.2016, p. 13-18.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biogeochemical dissolution of nontronite by Shewanella oneidensis MR-1

T2 - Evidence of biotic illite formation

AU - Koo, Tae Hee

AU - Lee, Gunjeong

AU - Kim, Jin-Wook

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Microbial Fe-reduction in smectite structure plays a significant role in illitization accompanying with the structural/chemical modification of smectite, closely linked to the physico-chemical properties of clays, Fe-liberation, water chemistry, elemental cycles, and fault behavior. Biotic dissolution of smectite is a major process that promotes illitization, however direct evidence of illite formation is not clearly understood. In the present study, a combination of spectroscopic, microscopic, and chemical analyses revealed evidence of illitization in bio-reduced smectite, with elemental composition measured on a nanoscale as incubation time increased. Fe-reducing bacteria (FeRB), Shewanella oneidensis MR-1 was inoculated in M1 medium with nontronite (NAu-1) less than 0.2 μm as an electron acceptor and Na-lactate as a sole electron donor at 30 °C in the anaerobic chamber for up to 120 days. The alkalinity was maintained at pH 8.0 in the whole experiment to enhance illite formation. The extent of Fe(III) reduction measured by 1,10-phenanthroline assay reached up to 10.6% in the experiment while less than ~ 1% of reduction was measured in no-bacteria control. In biotic and abiotic control, increases of elemental concentrations (Si, Al, and Fe) in the supernatant indicated the dissolution of nontronite. The progress of bio-reduced nontronite reaction can be explained as follows: altered nontronite (AN) with a scouring surface texture → K-nontronite (KN) with frayed edges → euhedral lath shaped illite. A progressive morphology change in bio-reduced nontronite corresponded to an increase in Al/Si and K / (K + 2Ca) that ranged between 0.13 and 0.28 and 0.16 to 1.0, suggesting the biotic reductive dissolution of nontronite and neoformation of illite. The precipitation of biotic amorphous silica supported the reductive dissolution of nontronite. In contrast, there was no clear evidence of mineral precipitation in no-bacteria control. Following treatment with Li and ethylene glycol for the long-term incubation (70 and 120 days), the X-ray diffraction (XRD) profiles confirmed illitization by displaying a 10-Å peak shoulder at around 8.9° 2θ in the bio-reduced nontronite. Indeed, a direct microscopic observation of distinct illite packets of 16 nm in thickness with d001 = 1.0 nm in the wavy nontronite matrix with various spacings (d001 = 1.2–1.3 nm) strongly suggested biotic illite formation.

AB - Microbial Fe-reduction in smectite structure plays a significant role in illitization accompanying with the structural/chemical modification of smectite, closely linked to the physico-chemical properties of clays, Fe-liberation, water chemistry, elemental cycles, and fault behavior. Biotic dissolution of smectite is a major process that promotes illitization, however direct evidence of illite formation is not clearly understood. In the present study, a combination of spectroscopic, microscopic, and chemical analyses revealed evidence of illitization in bio-reduced smectite, with elemental composition measured on a nanoscale as incubation time increased. Fe-reducing bacteria (FeRB), Shewanella oneidensis MR-1 was inoculated in M1 medium with nontronite (NAu-1) less than 0.2 μm as an electron acceptor and Na-lactate as a sole electron donor at 30 °C in the anaerobic chamber for up to 120 days. The alkalinity was maintained at pH 8.0 in the whole experiment to enhance illite formation. The extent of Fe(III) reduction measured by 1,10-phenanthroline assay reached up to 10.6% in the experiment while less than ~ 1% of reduction was measured in no-bacteria control. In biotic and abiotic control, increases of elemental concentrations (Si, Al, and Fe) in the supernatant indicated the dissolution of nontronite. The progress of bio-reduced nontronite reaction can be explained as follows: altered nontronite (AN) with a scouring surface texture → K-nontronite (KN) with frayed edges → euhedral lath shaped illite. A progressive morphology change in bio-reduced nontronite corresponded to an increase in Al/Si and K / (K + 2Ca) that ranged between 0.13 and 0.28 and 0.16 to 1.0, suggesting the biotic reductive dissolution of nontronite and neoformation of illite. The precipitation of biotic amorphous silica supported the reductive dissolution of nontronite. In contrast, there was no clear evidence of mineral precipitation in no-bacteria control. Following treatment with Li and ethylene glycol for the long-term incubation (70 and 120 days), the X-ray diffraction (XRD) profiles confirmed illitization by displaying a 10-Å peak shoulder at around 8.9° 2θ in the bio-reduced nontronite. Indeed, a direct microscopic observation of distinct illite packets of 16 nm in thickness with d001 = 1.0 nm in the wavy nontronite matrix with various spacings (d001 = 1.2–1.3 nm) strongly suggested biotic illite formation.

UR - http://www.scopus.com/inward/record.url?scp=84962003960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962003960&partnerID=8YFLogxK

U2 - 10.1016/j.clay.2016.03.030

DO - 10.1016/j.clay.2016.03.030

M3 - Article

VL - 134

SP - 13

EP - 18

JO - Applied Clay Science

JF - Applied Clay Science

SN - 0169-1317

ER -