Biological characterization of epigallocatechin gallate complex with different steviol glucosides

Thi Thanh Hanh Nguyen, Nahyun M. Kim, Su Cheong Yeom, Songhee Han, So Hyung Kwak, Seong Bo Kim, Jun Seong Park, Il Kyoon Mok, Doman Kim

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Steviol glucosides (SGs) such as rubusoside (Ru), stevioside (Ste), rebaudioside A (RebA) and stevioside glucosides (SG) are herbal tea sweeteners that enhance the solubility and stability of a number of pharmaceutically important compounds. The complex of epigallocatechin gallate (EGCG) with 10% (w/v) each Ru, Ste, RebA or SG enhanced the water solubility of EGCG over 15 times to 345, 312, 341, or 320 mg/mL, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging (SC50) activities of EGCG, EGCG-Ru, EGCG-Ste, EGCG-RebA, and EGCG-SG in water were 5.88, 6.03, 6.52, 4.89, and 4.23 μg/mL, respectively. EGCGs complexed with different SGs maintained inhibitory activities against human intestinal maltase, human pancreatic α-amylase, and the growth of Streptococcus mutans, Helicobacter pylori, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Clostridium difficile. In glucose tolerance test using C57BL/6 mice, plasma glucose levels in mice treated with EGCG or EGCG-Ste complex were decreased by 9.34%, which was 31.08% lower than those treated with maltose. The efficient and cost-effective EGCG-SGs production method might be applicable to produce water soluble bioactive nutraceuticals in large scale.

Original languageEnglish
Pages (from-to)512-517
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Issue number5
Publication statusPublished - 2017 Sep 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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  • Cite this

    Nguyen, T. T. H., Kim, N. M., Yeom, S. C., Han, S., Kwak, S. H., Kim, S. B., Park, J. S., Mok, I. K., & Kim, D. (2017). Biological characterization of epigallocatechin gallate complex with different steviol glucosides. Biotechnology and Bioprocess Engineering, 22(5), 512-517.