Biorecognition on graphene

Physical, covalent, and affinity immobilization methods exhibiting dramatic differences

Adeline Huiling Loo, Alessandra Bonanni, Martin Pumera

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The preparation of biorecognition layers on the surface of a sensing platform is a very crucial step for the development of sensitive and selective biosensors. Different protocols have been used thus far for the immobilization of biomolecules onto various electrode surfaces. In this work, we investigate how the protocol followed for the immobilization of a DNA aptamer affects the performance of the fabricated thrombin aptasensor. Specifically, the differences in selectivity and optimum amount of immobilized aptamer of the fabricated aptasensors adopting either physical, covalent, or affinity immobilization were compared. It was discovered that while all three methods of immobilization uniformly show a similar optimum amount of immobilized aptamer, physical, and covalent immobilization methods exhibit higher selectivity than affinity immobilization. Hence, it is believed that our findings are very important in order to optimize and improve the performance of graphene-based aptasensors.

Original languageEnglish
Pages (from-to)198-203
Number of pages6
JournalChemistry - An Asian Journal
Volume8
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

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Graphite
Immobilization
Nucleotide Aptamers
Biomolecules
Biosensors
Thrombin
Electrodes
Physical Restraint
Biosensing Techniques

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry
  • Organic Chemistry

Cite this

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Biorecognition on graphene : Physical, covalent, and affinity immobilization methods exhibiting dramatic differences. / Loo, Adeline Huiling; Bonanni, Alessandra; Pumera, Martin.

In: Chemistry - An Asian Journal, Vol. 8, No. 1, 01.01.2013, p. 198-203.

Research output: Contribution to journalArticle

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