Direct reprogramming is an efficient strategy to produce cardiac lineage cells necessary for cardiac tissue engineering and drug testing for cardiac toxicity. However, functional maturation of reprogrammed cardiomyocytes, which is of great importance for their regenerative potential and drug response, still remains challenging. In this study, we propose a novel electrode platform to promote direct cardiac reprogramming and improve the functionality of reprogrammed cardiac cells. Nonviral cardiac reprogramming was improved via a three-dimensional spheroid culture of chemically induced cardiomyocytes exposed to a small-molecule cocktail. A micropillar electrode array providing biphasic electrical pulses mimicking the heartbeat further enhanced maturation and electrophysiological properties of reprogrammed cardiac spheroids, leading to proper responses and increased sensitivity to drugs. On the basis of our results, we conclude that our device may have a wider application in the generation of functional cardiac cells for regenerative medicine and screening of novel drugs.
Bibliographical noteFunding Information:
This work was supported by a grant (19172MFDS168) funded by the Ministry of Food and Drug Safety (MFDS). This work was also supported by the National Research Foundation of Korea (NRF) grants funded by the Ministry of Science and ICT (MSIT) (2018R1D1A1B07042768 to Y.J., 2017R1A2B3011586 and 2018M3C7A1024654 to H.-J.C.). This work was supported by the Institute for Basic Science (IBS-R026-D1).
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering