Black phosphorus nanoparticles as a novel fluorescent sensing platform for nucleic acid detection

Ying Teng Yew, Zdeněk Sofer, Carmen C. Mayorga-Martinez, Martin Pumera

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Two-dimensional black phosphorus is an emerging material. For the past two years, black phosphorus has attracted the attention of the scientific community due to its promising applications in electronic devices and biosensors. In this work, black phosphorus nanoparticles (BPNPs) derived from a facile, one-step synthesis method were successfully employed as nanofluorophores in the development of a fluorescent sensing platform for DNA detection, for the first time. This detection strategy exploits the differences in affinities of labelled single-stranded and double-stranded DNA oligonucleotides towards spontaneous self-assembly with black phosphorus nanoparticles. A wide linear detection range with good linearity (r = 0.91) spanning from 4 pM to 4000 pM was achieved with the constructed nanosensor, including the ability to distinguish triple nucleotide polymorphism. Moreover, high sensitivity was also demonstrated with a low limit of detection (LOD = 5.9 pM) and limit of quantification (LOQ = 19.7 pM). This paper demonstrates a proof of concept that black phosphorus possesses promising potential for biosensing applications, in particular as DNA nanobiosensors, and the reported findings would be significant in encouraging further research work into the development of biomedical devices and technologies based on black phosphorus.

Original languageEnglish
Pages (from-to)1130-1136
Number of pages7
JournalMaterials Chemistry Frontiers
Volume1
Issue number6
DOIs
Publication statusPublished - 2017 Jun 1

Fingerprint

Nucleic acids
Phosphorus
Nucleic Acids
Nanoparticles
DNA
Nanosensors
Oligonucleotides
Nucleotides
Polymorphism
Biosensors
Self assembly

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Materials Chemistry

Cite this

Yew, Ying Teng ; Sofer, Zdeněk ; Mayorga-Martinez, Carmen C. ; Pumera, Martin. / Black phosphorus nanoparticles as a novel fluorescent sensing platform for nucleic acid detection. In: Materials Chemistry Frontiers. 2017 ; Vol. 1, No. 6. pp. 1130-1136.
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Black phosphorus nanoparticles as a novel fluorescent sensing platform for nucleic acid detection. / Yew, Ying Teng; Sofer, Zdeněk; Mayorga-Martinez, Carmen C.; Pumera, Martin.

In: Materials Chemistry Frontiers, Vol. 1, No. 6, 01.06.2017, p. 1130-1136.

Research output: Contribution to journalArticle

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