Blockade of oxidative stress by vitamin C ameliorates albuminuria and renal sclerosis in experimental diabetic rats

Eun Young Lee, Mi Young Lee, Soon Won Hong, Choon Hee Chung, Sae Yong Hong

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Purpose: Oxidative stress has been suggested to play a role as a common mediator of apoptosis and kidney damage in diabetes. However, it is uncertain whether the apoptosis occurs in the kidney during the course of diabetes. We investigated the occurrence of apoptosis in the diabetic rat kidney, the role of oxidative stress and the effect of an antioxidant on apoptosis in the diabetic rat kidney. Materials and Methods: Otsuka-Long-Evans-Tokushima-Fatty rats, an animal model for type 2 diabetes, were randomized into a non-treated diabetic (n = 8) and a vitamin C-treated group (n = 8). Long-Evans Tokushima Otsuka rats (n = 8) were used as a control. Results: Apoptosis was present in the epithelial cells of the proximal tubules in diabetic rats. The number of apoptotic cells, albuminuria, proteinuria, glomerular and tubulointerstitial sclerosis, and renal malondialdehyde were significantly decreased in vitamin C-treated diabetic rats when compared to the untreated diabetic rats. The decreased slit pore density (number of slit pores per underlying glomerular basement membrane length) as assessed by electron microscopy was also significantly restored by treatment with vitamin C without significantly affecting plasma glucose in diabetic rats. Conclusion: By blocking these pathophysiologic processes, a blockade of oxidative stress by vitamin C might become a useful adjunct to albuminuria and renal sclerosis in diabetic nephropathy.

Original languageEnglish
Pages (from-to)847-855
Number of pages9
JournalYonsei medical journal
Volume48
Issue number5
DOIs
Publication statusPublished - 2007 Oct 1

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Albuminuria
Sclerosis
Ascorbic Acid
Oxidative Stress
Kidney
Apoptosis
Inbred OLETF Rats
Glomerular Basement Membrane
Diabetic Nephropathies
Malondialdehyde
Proteinuria
Type 2 Diabetes Mellitus
Electron Microscopy
Animal Models
Cell Count
Antioxidants
Epithelial Cells
Glucose

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, Eun Young ; Lee, Mi Young ; Hong, Soon Won ; Chung, Choon Hee ; Hong, Sae Yong. / Blockade of oxidative stress by vitamin C ameliorates albuminuria and renal sclerosis in experimental diabetic rats. In: Yonsei medical journal. 2007 ; Vol. 48, No. 5. pp. 847-855.
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Blockade of oxidative stress by vitamin C ameliorates albuminuria and renal sclerosis in experimental diabetic rats. / Lee, Eun Young; Lee, Mi Young; Hong, Soon Won; Chung, Choon Hee; Hong, Sae Yong.

In: Yonsei medical journal, Vol. 48, No. 5, 01.10.2007, p. 847-855.

Research output: Contribution to journalArticle

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