Blu-ray based optomagnetic aptasensor for detection of small molecules

Jaeyoung Yang, Marco Donolato, Alessandro Pinto, Filippo Giacomo Bosco, En Te Hwu, Ching Hsiu Chen, Tommy Sonne Alstrøm, Gwan Hyoung Lee, Thomas Schäfer, Paolo Vavassori, Anja Boisen, Qiao Lin, Mikkel Fougt Hansen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This paper describes an aptamer-based optomagnetic biosensor for detection of a small molecule based on target binding-induced inhibition of magnetic nanoparticle (MNP) clustering. For the detection of a target small molecule, two mutually exclusive binding reactions (aptamer-target binding and aptamer-DNA linker hybridization) are designed. An aptamer specific to the target and a DNA linker complementary to a part of the aptamer sequence are immobilized onto separate MNPs. Hybridization of the DNA linker and the aptamer induces formation of MNP clusters. The target-to-aptamer binding on MNPs prior to the addition of linker-functionalized MNPs significantly hinders the hybridization reaction, thus reducing the degree of MNP clustering. The clustering state, which is thus related to the target concentration, is then quantitatively determined by an optomagnetic readout technique that provides the hydrodynamic size distribution of MNPs and their clusters. A commercial Blu-ray optical pickup unit is used for optical signal acquisition, which enables the establishment of a low-cost and miniaturized biosensing platform. Experimental results show that the degree of MNP clustering correlates well with the concentration of a target small molecule, adenosine triphosphate (ATP) in this work, in the range between 10. μM and 10. mM. This successful proof-of-concept indicates that our optomagnetic aptasensor can be further developed as a low-cost biosensing platform for detection of small molecule biomarkers in an out-of-lab setting.

Original languageEnglish
Pages (from-to)396-403
Number of pages8
JournalBiosensors and Bioelectronics
Volume75
DOIs
Publication statusPublished - 2016 Jan 15

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Nanoparticles
Cluster Analysis
Nucleotide Aptamers
Molecules
DNA
Costs and Cost Analysis
Pickups
Biosensing Techniques
Biomarkers
Hydrodynamics
Biosensors
Costs
Complementary DNA
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Yang, J., Donolato, M., Pinto, A., Bosco, F. G., Hwu, E. T., Chen, C. H., ... Hansen, M. F. (2016). Blu-ray based optomagnetic aptasensor for detection of small molecules. Biosensors and Bioelectronics, 75, 396-403. https://doi.org/10.1016/j.bios.2015.08.062
Yang, Jaeyoung ; Donolato, Marco ; Pinto, Alessandro ; Bosco, Filippo Giacomo ; Hwu, En Te ; Chen, Ching Hsiu ; Alstrøm, Tommy Sonne ; Lee, Gwan Hyoung ; Schäfer, Thomas ; Vavassori, Paolo ; Boisen, Anja ; Lin, Qiao ; Hansen, Mikkel Fougt. / Blu-ray based optomagnetic aptasensor for detection of small molecules. In: Biosensors and Bioelectronics. 2016 ; Vol. 75. pp. 396-403.
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Yang, J, Donolato, M, Pinto, A, Bosco, FG, Hwu, ET, Chen, CH, Alstrøm, TS, Lee, GH, Schäfer, T, Vavassori, P, Boisen, A, Lin, Q & Hansen, MF 2016, 'Blu-ray based optomagnetic aptasensor for detection of small molecules', Biosensors and Bioelectronics, vol. 75, pp. 396-403. https://doi.org/10.1016/j.bios.2015.08.062

Blu-ray based optomagnetic aptasensor for detection of small molecules. / Yang, Jaeyoung; Donolato, Marco; Pinto, Alessandro; Bosco, Filippo Giacomo; Hwu, En Te; Chen, Ching Hsiu; Alstrøm, Tommy Sonne; Lee, Gwan Hyoung; Schäfer, Thomas; Vavassori, Paolo; Boisen, Anja; Lin, Qiao; Hansen, Mikkel Fougt.

In: Biosensors and Bioelectronics, Vol. 75, 15.01.2016, p. 396-403.

Research output: Contribution to journalArticle

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T1 - Blu-ray based optomagnetic aptasensor for detection of small molecules

AU - Yang, Jaeyoung

AU - Donolato, Marco

AU - Pinto, Alessandro

AU - Bosco, Filippo Giacomo

AU - Hwu, En Te

AU - Chen, Ching Hsiu

AU - Alstrøm, Tommy Sonne

AU - Lee, Gwan Hyoung

AU - Schäfer, Thomas

AU - Vavassori, Paolo

AU - Boisen, Anja

AU - Lin, Qiao

AU - Hansen, Mikkel Fougt

PY - 2016/1/15

Y1 - 2016/1/15

N2 - This paper describes an aptamer-based optomagnetic biosensor for detection of a small molecule based on target binding-induced inhibition of magnetic nanoparticle (MNP) clustering. For the detection of a target small molecule, two mutually exclusive binding reactions (aptamer-target binding and aptamer-DNA linker hybridization) are designed. An aptamer specific to the target and a DNA linker complementary to a part of the aptamer sequence are immobilized onto separate MNPs. Hybridization of the DNA linker and the aptamer induces formation of MNP clusters. The target-to-aptamer binding on MNPs prior to the addition of linker-functionalized MNPs significantly hinders the hybridization reaction, thus reducing the degree of MNP clustering. The clustering state, which is thus related to the target concentration, is then quantitatively determined by an optomagnetic readout technique that provides the hydrodynamic size distribution of MNPs and their clusters. A commercial Blu-ray optical pickup unit is used for optical signal acquisition, which enables the establishment of a low-cost and miniaturized biosensing platform. Experimental results show that the degree of MNP clustering correlates well with the concentration of a target small molecule, adenosine triphosphate (ATP) in this work, in the range between 10. μM and 10. mM. This successful proof-of-concept indicates that our optomagnetic aptasensor can be further developed as a low-cost biosensing platform for detection of small molecule biomarkers in an out-of-lab setting.

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