A variety of biophysical and biochemical factors control stem cell differentiation. In this study, we developed a nanopatterned substrate platform to surface immobilize osteoinductive bone morphogenetic protein-2 (BMP-2) peptides. Specifically, polyurethane acrylate (PUA) substrates with nanometer-scale groove- and dot-shaped topography were fabricated. The nanopatterned PUA surface was uniformly coated with poly(glycidyl methacrylate) (pGMA) by initiated chemical vapor deposition (iCVD) followed by covalent immobilization of BMP-2 peptides. This approach resulted in much more efficient BMP-2 peptide immobilization than physical adsorption. The combined effects of biochemical signals from BMP-2 peptides and nanotopographical stimulation on osteogenic differentiation of hMSCs were examined in culture with and without soluble osteogenic factors. Results of Alizarin Red S staining, immunostaining, and quantitative real-time polymerase chain reaction revealed that hMSCs cultured on nanopatterned surfaces with immobilized BMP-2 peptides exhibited greater potential for osteogenic differentiation than hMSCs on a flat surface. Furthermore, the nanopatterned substrates with BMP-2 peptides directed osteogenic differentiation of hMSCs even without osteogenesis soluble inducing factors. Substrates with nanotopography and bioactive signals that induce differentiation of stem cells towards specific lineages could be used to develop functional stem cell culture substrates and tissue engineered scaffolds for therapeutic applications.
|Number of pages||11|
|Publication status||Published - 2013 Oct|
Bibliographical noteFunding Information:
This work was supported by a grant ( 2010-0022037 ) funded by the National Research Foundation of Korea , the Ministry of Education, Science and Technology, Republic of Korea. This work was also supported by a grant ( A110552 ) from the Korea Health Technology R&D Project of the Ministry of Health & Welfare of the Republic of Korea. This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Education, Science and Technology ( ABC-2011-0031356 ), and by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( 2012-0003282 ).
All Science Journal Classification (ASJC) codes
- Ceramics and Composites
- Mechanics of Materials