BMP-2 peptide-functionalized nanopatterned substrates for enhanced osteogenic differentiation of human mesenchymal stem cells

Mun Jung Kim, Bora Lee, Kisuk Yang, Junyong Park, Seokwoo Jeon, Soong Ho Um, Dong Ik Kim, Sung Gap Im, Seung-Woo Cho

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

A variety of biophysical and biochemical factors control stem cell differentiation. In this study, we developed a nanopatterned substrate platform to surface immobilize osteoinductive bone morphogenetic protein-2 (BMP-2) peptides. Specifically, polyurethane acrylate (PUA) substrates with nanometer-scale groove- and dot-shaped topography were fabricated. The nanopatterned PUA surface was uniformly coated with poly(glycidyl methacrylate) (pGMA) by initiated chemical vapor deposition (iCVD) followed by covalent immobilization of BMP-2 peptides. This approach resulted in much more efficient BMP-2 peptide immobilization than physical adsorption. The combined effects of biochemical signals from BMP-2 peptides and nanotopographical stimulation on osteogenic differentiation of hMSCs were examined in culture with and without soluble osteogenic factors. Results of Alizarin Red S staining, immunostaining, and quantitative real-time polymerase chain reaction revealed that hMSCs cultured on nanopatterned surfaces with immobilized BMP-2 peptides exhibited greater potential for osteogenic differentiation than hMSCs on a flat surface. Furthermore, the nanopatterned substrates with BMP-2 peptides directed osteogenic differentiation of hMSCs even without osteogenesis soluble inducing factors. Substrates with nanotopography and bioactive signals that induce differentiation of stem cells towards specific lineages could be used to develop functional stem cell culture substrates and tissue engineered scaffolds for therapeutic applications.

Original languageEnglish
Pages (from-to)7236-7246
Number of pages11
JournalBiomaterials
Volume34
Issue number30
DOIs
Publication statusPublished - 2013 Oct 1

Fingerprint

Bone Morphogenetic Protein 2
Stem cells
Mesenchymal Stromal Cells
Peptides
Bone
Proteins
Substrates
Polyurethanes
Stem Cells
Alizarin
Physical Restraint
Immobilized Proteins
Tissue Scaffolds
Polymerase chain reaction
Cell culture
Osteogenesis
Immobilization
Topography
Adsorption
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Kim, Mun Jung ; Lee, Bora ; Yang, Kisuk ; Park, Junyong ; Jeon, Seokwoo ; Um, Soong Ho ; Kim, Dong Ik ; Im, Sung Gap ; Cho, Seung-Woo. / BMP-2 peptide-functionalized nanopatterned substrates for enhanced osteogenic differentiation of human mesenchymal stem cells. In: Biomaterials. 2013 ; Vol. 34, No. 30. pp. 7236-7246.
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BMP-2 peptide-functionalized nanopatterned substrates for enhanced osteogenic differentiation of human mesenchymal stem cells. / Kim, Mun Jung; Lee, Bora; Yang, Kisuk; Park, Junyong; Jeon, Seokwoo; Um, Soong Ho; Kim, Dong Ik; Im, Sung Gap; Cho, Seung-Woo.

In: Biomaterials, Vol. 34, No. 30, 01.10.2013, p. 7236-7246.

Research output: Contribution to journalArticle

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