Abstract
Tooth morphogenesis is regulated by sequential and reciprocal interaction between oral epithelium and neural-crest-derived ectomesenchyme. The interaction is controlled by various signal molecules such as bone morphogenetic protein (BMP), Hedgehog, Wbroblast growth factor (FGF), and Wnt. Zeb family is known as a transcription factor, which is essential for neural development and neural-crest-derived tissues, whereas the role of the Zeb family in tooth development remains unclear. Therefore, this study aimed to investigate the expression proWles of Zeb1 and Zeb2 during craniofacial development focusing on mesenchyme of palate, hair follicle, and tooth germ from E12.5 to E16.5. In addition, we examined the interaction between Zeb family and BMP4 during tooth development. Both Zeb1 and Zeb2 were expressed at mesenchyme of the palate, hair follicle, and tooth germ throughout the stages. In the case of tooth germ at the cap stage, the expression of Zeb1 and Zeb2 was lost in epithelium-separated dental mesenchyme. However, the expression of Zeb1 and Zeb2 in the dental mesenchyme was recovered by Bmp4 signaling via BMP4-soaked bead and tissue recombination. Our results suggest that Zeb1 and Zeb2, which were mediated by BMP4, play an important role in neural-crestderived craniofacial organ morphogenesis, such as tooth development.
| Original language | English |
|---|---|
| Pages (from-to) | 791-800 |
| Number of pages | 10 |
| Journal | Histochemistry and cell biology |
| Volume | 137 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2012 Jun 1 |
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All Science Journal Classification (ASJC) codes
- Histology
- Molecular Biology
- Medical Laboratory Technology
- Cell Biology
Cite this
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BMP4 signaling mediates Zeb family in developing mouse tooth. / Shin, Jeong Oh; Kim, Eun Jung; Cho, Kyoung Won; Nakagawa, Eizo; Kwon, Hyuk Jae; Cho, Sung Won; Jung, Han Sung.
In: Histochemistry and cell biology, Vol. 137, No. 6, 01.06.2012, p. 791-800.Research output: Contribution to journal › Article
TY - JOUR
T1 - BMP4 signaling mediates Zeb family in developing mouse tooth
AU - Shin, Jeong Oh
AU - Kim, Eun Jung
AU - Cho, Kyoung Won
AU - Nakagawa, Eizo
AU - Kwon, Hyuk Jae
AU - Cho, Sung Won
AU - Jung, Han Sung
PY - 2012/6/1
Y1 - 2012/6/1
N2 - Tooth morphogenesis is regulated by sequential and reciprocal interaction between oral epithelium and neural-crest-derived ectomesenchyme. The interaction is controlled by various signal molecules such as bone morphogenetic protein (BMP), Hedgehog, Wbroblast growth factor (FGF), and Wnt. Zeb family is known as a transcription factor, which is essential for neural development and neural-crest-derived tissues, whereas the role of the Zeb family in tooth development remains unclear. Therefore, this study aimed to investigate the expression proWles of Zeb1 and Zeb2 during craniofacial development focusing on mesenchyme of palate, hair follicle, and tooth germ from E12.5 to E16.5. In addition, we examined the interaction between Zeb family and BMP4 during tooth development. Both Zeb1 and Zeb2 were expressed at mesenchyme of the palate, hair follicle, and tooth germ throughout the stages. In the case of tooth germ at the cap stage, the expression of Zeb1 and Zeb2 was lost in epithelium-separated dental mesenchyme. However, the expression of Zeb1 and Zeb2 in the dental mesenchyme was recovered by Bmp4 signaling via BMP4-soaked bead and tissue recombination. Our results suggest that Zeb1 and Zeb2, which were mediated by BMP4, play an important role in neural-crestderived craniofacial organ morphogenesis, such as tooth development.
AB - Tooth morphogenesis is regulated by sequential and reciprocal interaction between oral epithelium and neural-crest-derived ectomesenchyme. The interaction is controlled by various signal molecules such as bone morphogenetic protein (BMP), Hedgehog, Wbroblast growth factor (FGF), and Wnt. Zeb family is known as a transcription factor, which is essential for neural development and neural-crest-derived tissues, whereas the role of the Zeb family in tooth development remains unclear. Therefore, this study aimed to investigate the expression proWles of Zeb1 and Zeb2 during craniofacial development focusing on mesenchyme of palate, hair follicle, and tooth germ from E12.5 to E16.5. In addition, we examined the interaction between Zeb family and BMP4 during tooth development. Both Zeb1 and Zeb2 were expressed at mesenchyme of the palate, hair follicle, and tooth germ throughout the stages. In the case of tooth germ at the cap stage, the expression of Zeb1 and Zeb2 was lost in epithelium-separated dental mesenchyme. However, the expression of Zeb1 and Zeb2 in the dental mesenchyme was recovered by Bmp4 signaling via BMP4-soaked bead and tissue recombination. Our results suggest that Zeb1 and Zeb2, which were mediated by BMP4, play an important role in neural-crestderived craniofacial organ morphogenesis, such as tooth development.
UR - http://www.scopus.com/inward/record.url?scp=84865122868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865122868&partnerID=8YFLogxK
U2 - 10.1007/s00418-012-0930-7
DO - 10.1007/s00418-012-0930-7
M3 - Article
C2 - 22350174
AN - SCOPUS:84865122868
VL - 137
SP - 791
EP - 800
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 6
ER -