Bone cell response to fluid shear stress and cyclic compressive strain in 3D trabecular bone

Byung Gwan Kim, Ji Hyun Kwag, Han Sung Kim, Kyung Hwan Kim, Chi Hyun Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mechanical loading is a critical factor that regulate functional bone adaptation and trabecular bone architecture is dependent on the mechanical environment within bone tissue. The aim of this study is to investigate the bone cell response to mechanical loading in a 3D trabecular bone environment using two different bone cell loading mechanism. We established a 3D trabecular bone explant model and applied either oscillatory fluid flow induced shear stress or cyclic compressive strain. Our results indicate that fluid shear stress increases alkaline phosphatase activity as well as mRNA levels of collagen type I and core binding factor 1. Mechanical compressive strain also increases alkaline phosphatase activity and collagen type I mRNA but to a lesser degree compared to fluid shear stress. These results suggest that fluid shear stress may be a more potent stimulus than cyclic compressive strain in inducing bone formation in 3D trabecular bone structure.

Original languageEnglish
Pages (from-to)540-548
Number of pages9
JournalTissue Engineering and Regenerative Medicine
Volume6
Issue number4-11
Publication statusPublished - 2009 Jun 1

Fingerprint

Shear stress
Bone
Bone and Bones
Fluids
Collagen Type I
Alkaline Phosphatase
Core Binding Factors
Messenger RNA
Phosphatases
Osteogenesis
Collagen
Cancellous Bone
Flow of fluids
Tissue

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

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abstract = "Mechanical loading is a critical factor that regulate functional bone adaptation and trabecular bone architecture is dependent on the mechanical environment within bone tissue. The aim of this study is to investigate the bone cell response to mechanical loading in a 3D trabecular bone environment using two different bone cell loading mechanism. We established a 3D trabecular bone explant model and applied either oscillatory fluid flow induced shear stress or cyclic compressive strain. Our results indicate that fluid shear stress increases alkaline phosphatase activity as well as mRNA levels of collagen type I and core binding factor 1. Mechanical compressive strain also increases alkaline phosphatase activity and collagen type I mRNA but to a lesser degree compared to fluid shear stress. These results suggest that fluid shear stress may be a more potent stimulus than cyclic compressive strain in inducing bone formation in 3D trabecular bone structure.",
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Bone cell response to fluid shear stress and cyclic compressive strain in 3D trabecular bone. / Kim, Byung Gwan; Kwag, Ji Hyun; Kim, Han Sung; Kim, Kyung Hwan; Kim, Chi Hyun.

In: Tissue Engineering and Regenerative Medicine, Vol. 6, No. 4-11, 01.06.2009, p. 540-548.

Research output: Contribution to journalArticle

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AU - Kwag, Ji Hyun

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AU - Kim, Kyung Hwan

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